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Sample GSM2909395 Query DataSets for GSM2909395
Status Public on Feb 12, 2024
Title Non-tumor adult A2B5+ WM, sample 2
Sample type RNA
Source name Dissociated cells from normal adult WM, sorted on A2B5
Organism Homo sapiens
Characteristics tumor type: non-tumor (normal)
tumor grade: non-tumor (normal)
cell sort marker: A2B5+
developmental stage: adult
tissue source: white matter
Treatment protocol Tissue samples were dissociated with papain, cultured in suspension plates in serum-free media, and sorted MACS and FACS on A2B5 (tumor, adult normal samples) or CD140a (fetal normal samples).
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol miRNA was extracted and purified with the Qiagen miRNeasy kit, according to the manufacturer's instructions.
Label biotin
Label protocol Biotin labeling was performed according to the manufacturer's instructions.
Hybridization protocol Hybridization was performed according to the manufacturer's instructions.
Scan protocol GeneChip scanning and image processing were performed according to the manufacturer's instructions.
Description A2B5_ADULT_WM_NONTUMOR_2
Small RNA
miRNA expression from Dissociated cells from normal adult WM, sorted on A2B5.
Data processing Raw intensity values were normalized using Variance Stabilizing Normalization (VSN) with RMA-style probeset summarization, as implemented by the vsnrma function of the VSN package in Bioconductor, in the R statistical software environment. As the Affymetrix miRNA 3.0 array platform includes miRNA for multiple species, prior to differential expression analysis data was filtered to include probes corresponding to mature human miRNAs.
Submission date Jan 02, 2018
Last update date Feb 12, 2024
Contact name Steven Goldman
Organization name University of Rochester Medical Center
Department Center for Translational Neuromedicine
Lab Goldman Lab
Street address 601 Elmwood Ave
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
Platform ID GPL16384
Series (1)
GSE255589 Human Adult Glial Progenitors Express Distinct miRNA Profiles Compared to their Fetal Counterparts

Data table header descriptions
VALUE Log2 intensity, after normalization with VSN and RMA-style background correction

Data table
14q0_st 2.899395203
14qI-1_st 4.425203463
14qI-1_x_st 3.272972539
14qI-2_st 2.296802004
14qI-3_x_st 2.441469172
14qI-4_st 3.359883736
14qI-4_x_st 2.932226168
14qI-5_st 2.899395203
14qI-6_st 1.410569758
14qI-6_x_st 1.942478186
14qI-7_st 4.476359252
14qI-8_st 2.898464323
14qI-8_x_st 2.477610991
14qI-9_x_st 2.371262161
14qII-1_st 4.296632798
14qII-1_x_st 4.361833107
14qII-10_st 2.170779804
14qII-10_x_st 2.266539523
14qII-11_st 1.683978145
14qII-11_x_st 2.107668289

Total number of rows: 25119

Table truncated, full table size 710 Kbytes.

Supplementary file Size Download File type/resource
GSM2909395_A2B5_ADULT_WM_NONTUMOR_2.CEL.gz 770.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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