|
Status |
Public on Jul 01, 2008 |
Title |
NuclearRNA_20min_D |
Sample type |
RNA |
|
|
Source name |
FGF 20 min
|
Organism |
Mus musculus |
Characteristics |
NIH3T3 cell
|
Treatment protocol |
Serum starvation 36 h, 50 ng/ml FGF 20 min
|
Growth protocol |
NIH3T3 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% calf serum.
|
Extracted molecule |
nuclear RNA |
Extraction protocol |
Nuclear RNA was isolated from buffer RLN-lysed pellet using a Qiagen RNeasy kit (see Qiagen's supplementary protocol). On-column DNase digestion. The rest of the protocols were adopted from Affymetrix WT double-stranded target assay manual (SuperScript III RT at 50 °C instead of SuperScript II RT at 42°C).
|
Label |
Biotin
|
Label protocol |
Standard Affymetrix protocol (WT double stranded DNA terminal labeling)
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol
|
Description |
RNA mapping with nuclear RNA, FGF 20 min, Affymetrix Mouse Tiling 2.0R D array
|
Data processing |
Bar files were created using Affymetrix Tiling SDK release2 (BPMAP = NCBIv35, Quantile normalization, Bandwidth = 250, Signal scale = log2).
|
|
|
Submission date |
May 28, 2008 |
Last update date |
May 28, 2008 |
Contact name |
Eisuke Nishida |
E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
Phone |
+81-75-753-4230
|
Organization name |
Graduate School of Biostudies, Kyoto University
|
Department |
Department of Cell and Developmental Biology
|
Street address |
Kitashirakawa, Sakyo-ku
|
City |
Kyoto |
ZIP/Postal code |
606-8502 |
Country |
Japan |
|
|
Platform ID |
GPL6447 |
Series (1) |
GSE11576 |
RNA mapping and ChIP with tiling arrays in FGF-stimulated NIH3T3 cells |
|