|
| Status |
Public on Jan 17, 2018 |
| Title |
DM-2 |
| Sample type |
SRA |
| |
|
| Source name |
chicken embryo myoblast
|
| Organism |
Gallus gallus |
| Characteristics |
cell type: chicken embryo skeletal muscle derived myotubes
developmental stage: differentiated myotube
passages: 1
|
| Treatment protocol |
chicken primary myoblasts were induced to differentiation by culturing in differentiation medium (RPMI-1640, 2% horse serum and 2% pen/strep)
|
| Growth protocol |
chicken primary myoblasts were growed in growth medium containing RPMI-1640, 15% FBS and 2% pen/srep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of the proliferating myoblasts or differentiated myotubes were harvested using Trizol reagent according to the manufacturer’s direction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to gga5 whole genome using HISAT with default parameters.
Expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) were calculated using HTSeq.
Genome_build: Gallus_gallus-5.0
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Jan 16, 2018 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Wen Luo |
| E-mail(s) |
luowen729@scau.edu.cn
|
| Organization name |
South China Agricultural University
|
| Street address |
Wushan
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510642 |
| Country |
China |
| |
|
| Platform ID |
GPL24517 |
| Series (1) |
| GSE109225 |
RNA-sequencing of chicken primary myoblasts and myotubes |
|
| Relations |
| BioSample |
SAMN08367450 |
| SRA |
SRX3585087 |
