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Sample GSM2935576 Query DataSets for GSM2935576
Status Public on Jan 17, 2018
Title DM-3
Sample type SRA
 
Source name chicken embryo myoblast
Organism Gallus gallus
Characteristics cell type: chicken embryo skeletal muscle derived myotubes
developmental stage: differentiated myotube
passages: 1
Treatment protocol chicken primary myoblasts were induced to differentiation by culturing in differentiation medium (RPMI-1640, 2% horse serum and 2% pen/strep)
Growth protocol chicken primary myoblasts were growed in growth medium containing RPMI-1640, 15% FBS and 2% pen/srep
Extracted molecule total RNA
Extraction protocol Total RNA of the proliferating myoblasts or differentiated myotubes were harvested using Trizol reagent according to the manufacturer’s direction.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to gga5 whole genome using HISAT with default parameters.
Expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) were calculated using HTSeq.
Genome_build: Gallus_gallus-5.0
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
 
Submission date Jan 16, 2018
Last update date Jan 24, 2018
Contact name Wen Luo
E-mail(s) luowen729@scau.edu.cn
Organization name South China Agricultural University
Street address Wushan
City Guangzhou
State/province Guangdong
ZIP/Postal code 510642
Country China
 
Platform ID GPL24517
Series (1)
GSE109225 RNA-sequencing of chicken primary myoblasts and myotubes
Relations
BioSample SAMN08367449
SRA SRX3585088

Supplementary file Size Download File type/resource
GSM2935576_DM-3-FPKM.txt.gz 647.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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