|
| Status |
Public on May 29, 2019 |
| Title |
Root of control (untreated) Brassica napus, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Root of Brassica napus, left untreated for 4 days
|
| Organism |
Brassica napus |
| Characteristics |
strain: Nourin No.16
agent: none
tissue: root
growth stage: 4 weeks after germination
|
| Treatment protocol |
Plants were exposed to glutathione for 4 days. GSH (glutathione, reduced form) was applied to source leaves (fully expanded leaves). In the leaf GSH treatment, 50 µL of 100 mM GSH in a foliar-application solution (10 mM MES-KOH, pH6.1, 0.01% [w/v] Triton X-100) was applied to each leaf with a painting brush; GSH was applied to 3 expanded leaves per plant daily before harvest.
|
| Growth protocol |
Seeds of oilseed rape (Brassica napus L. cv. Nourin No.16, Kaneko Seed Co. Ltd., Gumma, Japan) were germinated in vermiculite by using bottom irrigation. Modified Hoagland solution (nutrient solution), described previously [Soil Sci. Plant Nutr. 54 (2008) 118–127.], was used as the irrigation solution. At 10 days after germination, young seedlings were transferred to 1.5-L containers (4 seedlings/container) containing 1.2 L of the nutrient solution, and the containers were maintained in a growth chamber for a 16-h photoperiod (~200μmol m-2s-1) at 24 °C, and at 16°C at night. Nutrient solutions were aerated continuously and renewed twice weekly. Each tested plant was grown for 4 weeks before being harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy Plant kit (Qiagen K.K., Tokyo, Japan).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was obtained from 100 ng of purified total RNA using a GeneChip 3’ IVT Express Kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Arabidopsis ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
No additional information
|
| Data processing |
CEL files were quantified with Distribution Free Weighted method (DFW), using the statistical language R (R-2.7.2) and Bioconductor 2.2.
|
| |
|
| Submission date |
Jan 17, 2018 |
| Last update date |
May 29, 2019 |
| Contact name |
Yuji Nakai |
| Organization name |
Hirosaki University
|
| Department |
Institute of Regional Innovation
|
| Lab |
Section of Food Sciences
|
| Street address |
2-1-1, Yanagawa
|
| City |
Aomori |
| State/province |
Aomori |
| ZIP/Postal code |
038-0012 |
| Country |
Japan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE109282 |
Effect of foliar GSH treatment on gene expression profile in the root of Brassica napus |
|
