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Sample GSM2948305 Query DataSets for GSM2948305
Status Public on Jan 25, 2019
Title MM.1S_drug
Sample type SRA
 
Source name multiple myeloma
Organism Homo sapiens
Characteristics tissue: peripheral blood
cell type: B lymphoblast
treatment: 72 h after OR-S1 treatment
Treatment protocol MM cells were treated with 1 μM OR-S1 for 72 h.
Growth protocol RPMI1640 supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy Mini Kit (Qiagen).
Libraries were prepared for sequencing using standard Illumina protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Avadis NGS Version 2.1 software was used for analysis.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using Avadis NGS Version 2.1.
Genome_build: hg19
 
Submission date Jan 25, 2018
Last update date Jan 25, 2019
Contact name Makoto Nakagawa
E-mail(s) maknakag@ncc.go.jp
Organization name National Cancer Center Research Institute
Department Division of Hematological Malignancy
Street address 5-1-1, Tsukiji, Chuo-ku
City Tokyo
ZIP/Postal code 104-0045
Country Japan
 
Platform ID GPL18573
Series (2)
GSE109673 Dual inhibition of EZH1/2 depletes stem cells and over-activates WNT signaling in multiple myeloma [RNA-seq]
GSE109675 Dual inhibition of EZH1/2 depletes stem cells and over-activates WNT signaling in multiple myeloma
Relations
BioSample SAMN08397416
SRA SRX3600905

Supplementary file Size Download File type/resource
GSM2948305_MM.1S_drug.txt.gz 900.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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