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Sample GSM29565 Query DataSets for GSM29565
Status Public on Aug 25, 2005
Title Donor 2 vg 0 hr
Sample type RNA
 
Source name T-cells, peripheral blood
Organism Homo sapiens
Extracted molecule total RNA
 
Description Peripheral blood leukocytes of three human donors were isolated from blood bank buffy-coat preparations by Ficoll-Hypaque density gradient centrifugation, and T-cells were purified with high affinity negative selection human CD3+ T-cell enrichment columns. The cells were resuspended in RPMI-1640 with 10% FCS with approximately 3-8 millions cells/mL at room temperature overnight. T-cells were then either unactivated (1g 0 hr), activated (1g 4 hr) with a final concentration of 5 mg/mL Con A + 4 mg/mL anti-CD28 antibody and incubated for 4 hours at 37°C, or loaded onto a RPM rotating at 60°/s. T-cells loaded on the RPM were cultured for 2 hours prior to collection or activation to allow equilibration of cells to the new environment. After incubation, 1 mL of 4 M guanidinium isothiocyanate in 750 mM sodium citrate buffer, pH 7, with N-lauroylsarcosine and ß-mercaptoethanol was added to lyse cells and stabilize RNA. Lysates were frozen at -80°C.
Lot batch = 2001996
 
Submission date Aug 25, 2004
Last update date Oct 28, 2005
Contact name Jim Boonyaratanakornkit
E-mail(s) jim.boonyaratanakornkit@gmail.com
Phone 301-594-2589
Organization name NIH - NIAID
Department LID
Lab RVS
Street address 50 South Dr, Bldg 50, Rm 6513
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL201
Series (1)
GSE1709 STS107 T-cell activation at 1g vs. vg

Data table header descriptions
ID_REF
Stat Pairs The number of probe pairs in the probe set.
Stat Pairs Used The number of probe pairs in the probe set used to assign a detection call.
VALUE A median normalized quantitative measure of the relative abundance of a transcript.
ABS_CALL A qualitative measurement indicating if the transcript is detected (Present), not detected (Absent), or marginally detected (Marginal).
DETECTION P-VALUE A p-value indicating the significance of the Detection call. A Detection p-value measures the probability that the discrimination scores of all probe pairs in the probe set are above a threshold and that the target transcript is likely to be present.

Data table
ID_REF Stat Pairs Stat Pairs Used VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 16 16 1.404762 A 0.418069
1053_at 16 16 0.16666667 A 0.732537
117_at 16 16 7.619048 P 0.006532
121_at 16 16 9.309524 P 0.017001
1255_g_at 16 16 0.71428573 A 0.438361
1294_at 16 16 6.690477 P 0.011447
1316_at 16 16 2.1904762 A 0.107301
1320_at 16 16 0.85714287 A 0.397994
1431_at 16 16 0.5 A 0.52062
1438_at 16 16 0.6666667 A 0.5
1487_at 16 16 2.2380953 A 0.339558
1494_f_at 16 16 2.3333335 A 0.081337
1598_g_at 16 16 3.5000002 A 0.35869
160020_at 16 16 2.7142859 A 0.07383
1729_at 16 16 1.5000001 A 0.602006
177_at 16 16 2.1190476 A 0.07383
1773_at 16 16 0.26190478 A 0.810313
1861_at 16 16 0.8333334 A 0.660442
200000_s_at 11 11 18.738096 P 0.000732
200001_at 11 11 2.809524 A 0.466064

Total number of rows: 8793

Table truncated, full table size 324 Kbytes.




Supplementary file Size Download File type/resource
GSM29565.CEL.gz 1.1 Mb (ftp)(http) CEL
GSM29565.EXP.gz 485 b (ftp)(http) EXP
GSM29565.rpt.gz 810 b (ftp)(http) RPT

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