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Sample GSM2974957 Query DataSets for GSM2974957
Status Public on Sep 30, 2018
Title RNA-seq_rep1
Sample type SRA
 
Source name seedling
Organism Arabidopsis thaliana
Characteristics cultivar: Col-0
tissue: seedling
age: 12 days
Growth protocol The Arabidopsis (Col-0 ecotype) seeds were germinated and grown in 1/2 MS for 12 days at 20°C under 16 hour light/8 hour dark.
Extracted molecule polyA RNA
Extraction protocol RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Sequenced reads were trimmed for adaptor sequence, and masked for low-quality sequence, then mapped to TAIR10 whole genome using tophat.
Genome_build: TAIR10
Supplementary_files_format_and_content: tab-delimited text files include the position information of RNA Pol II for each sample
Supplementary_files_format_and_content: bedgraph
 
Submission date Feb 01, 2018
Last update date Oct 31, 2018
Contact name Zhicheng Dong
E-mail(s) zc_dong@gzhu.edu.cn
Organization name Guangzhou University
Department School of Life Sciences
Street address 230 Wai Huan Xi Road,Guangzhou Higher Education Mega Center
City Guangzhou
State/province Guangdong
ZIP/Postal code 510006
Country China
 
Platform ID GPL13222
Series (1)
GSE109974 RNA polymerase II activity revealed by GRO-seq and pNET-seq in Arabidopsis
Relations
BioSample SAMN08450253
SRA SRX3638185

Supplementary file Size Download File type/resource
GSM2974957_RNA-seq_rep1.minus.bedgraph.gz 13.7 Mb (ftp)(http) BEDGRAPH
GSM2974957_RNA-seq_rep1.plus.bedgraph.gz 13.7 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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