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Sample GSM2974957 Query DataSets for GSM2974957
Status Public on Sep 30, 2018
Title RNA-seq_rep1
Sample type SRA
Source name seedling
Organism Arabidopsis thaliana
Characteristics cultivar: Col-0
tissue: seedling
age: 12 days
Growth protocol The Arabidopsis (Col-0 ecotype) seeds were germinated and grown in 1/2 MS for 12 days at 20°C under 16 hour light/8 hour dark.
Extracted molecule polyA RNA
Extraction protocol RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing Sequenced reads were trimmed for adaptor sequence, and masked for low-quality sequence, then mapped to TAIR10 whole genome using tophat.
Genome_build: TAIR10
Supplementary_files_format_and_content: tab-delimited text files include the position information of RNA Pol II for each sample
Supplementary_files_format_and_content: bedgraph
Submission date Feb 01, 2018
Last update date Oct 31, 2018
Contact name Zhicheng Dong
Organization name Guangzhou University
Department School of Life Sciences
Street address 230 Wai Huan Xi Road,Guangzhou Higher Education Mega Center
City Guangzhou
State/province Guangdong
ZIP/Postal code 510006
Country China
Platform ID GPL13222
Series (1)
GSE109974 RNA polymerase II activity revealed by GRO-seq and pNET-seq in Arabidopsis
BioSample SAMN08450253
SRA SRX3638185

Supplementary file Size Download File type/resource
GSM2974957_RNA-seq_rep1.minus.bedgraph.gz 13.7 Mb (ftp)(http) BEDGRAPH 13.7 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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