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Sample GSM2991904 Query DataSets for GSM2991904
Status Public on Feb 04, 2021
Title Fed Knockout 5
Sample type RNA
 
Source name Mouse intestines without PC synthesis in the re-fed state
Organism Mus musculus
Characteristics tissue: Intestine
genotpye: Pcyt1aLoxP/LoxP;VilCreERT2
age and sex: 10-12 week old female
Treatment protocol The mice were placed on a high fat diet for 3 days before being fasted overnight and re-fed a high fat diet for 2 hours. Intestines were excised and opened longitudinally. 3 cm was removed from the geometric middle of the small intetsine (jejunum) and stored in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction folllowed by RNeasy column clean-up.
Label Biotin
Label protocol Biotin labeled cRNA were prepared according to the standard Affymetrix WT Plus Kit Protocol from 400ng total RNA.
 
Hybridization protocol 16-18 hours at 45 degrees Celcius as descibed in Affymetrix protocol.
Scan protocol Affymetrix GeneChip Scanner 3000
Description Gene expression data from mouse intestines without PC synthesis in the re-fed state
Pcyt1aLoxp/LoxP;VilcreERT2 mice were treated with tamoxifen for 5 days to induce Cre recombinase, and LoxP/LoxP control mice were also treated with tamoxifen.
Data processing Expression Console 1.4.1.46 using RMA normalization; TAC 3
 
Submission date Feb 12, 2018
Last update date Feb 04, 2021
Contact name René L Jacobs
E-mail(s) rjacobs@ualberta.ca
Organization name University of Alberta
Department Agriculture, Food and Nutritional Science; Biochemistry
Lab Jacobs Lab
Street address Li Ka Shing Centre for Health Research
City Edmonton
State/province Alberta
ZIP/Postal code T6G2E1
Country Canada
 
Platform ID GPL16570
Series (1)
GSE110474 Expression data from intestine samples of mice lacking intestinal Pcyt1a, which encodes an enzyme that regulates phosphatidylcholine synthesis.

Data table header descriptions
ID_REF
VALUE Expression Console RMA, Linear signal

Data table
ID_REF VALUE
17200001 115.5
17200003 129.3
17200005 56.7
17200007 33.9
17200009 119.4
17200011 41.7
17200013 35.4
17200015 92.3
17200017 17.3
17200019 24.0
17200021 68.6
17200023 109.9
17200025 50.5
17200027 147.6
17200029 38.1
17200031 25.0
17200033 24.3
17200035 32.5
17200037 104.6
17200039 13.3

Total number of rows: 41345

Table truncated, full table size 574 Kbytes.




Supplementary file Size Download File type/resource
GSM2991904_FedKO5.CEL.gz 8.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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