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Sample GSM2991908 Query DataSets for GSM2991908
Status Public on Feb 04, 2021
Title Fed Control 4
Sample type RNA
 
Source name Mouse intestines with PC synthesis in the re-fed state
Organism Mus musculus
Characteristics tissue: Intestine
genotpye: Pcyt1aLoxP/LoxP
age and sex: 10-12 week old female
Treatment protocol The mice were placed on a high fat diet for 3 days before being fasted overnight and re-fed a high fat diet for 2 hours. Intestines were excised and opened longitudinally. 3 cm was removed from the geometric middle of the small intetsine (jejunum) and stored in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction folllowed by RNeasy column clean-up.
Label Biotin
Label protocol Biotin labeled cRNA were prepared according to the standard Affymetrix WT Plus Kit Protocol from 400ng total RNA.
 
Hybridization protocol 16-18 hours at 45 degrees Celcius as descibed in Affymetrix protocol.
Scan protocol Affymetrix GeneChip Scanner 3000
Description Gene expression data from mouse intestines with PC synthesis in the re-fed state
Pcyt1aLoxp/LoxP;VilcreERT2 mice were treated with tamoxifen for 5 days to induce Cre recombinase, and LoxP/LoxP control mice were also treated with tamoxifen.
Data processing Expression Console 1.4.1.46 using RMA normalization; TAC 3
 
Submission date Feb 12, 2018
Last update date Feb 04, 2021
Contact name René L Jacobs
E-mail(s) rjacobs@ualberta.ca
Organization name University of Alberta
Department Agriculture, Food and Nutritional Science; Biochemistry
Lab Jacobs Lab
Street address Li Ka Shing Centre for Health Research
City Edmonton
State/province Alberta
ZIP/Postal code T6G2E1
Country Canada
 
Platform ID GPL16570
Series (1)
GSE110474 Expression data from intestine samples of mice lacking intestinal Pcyt1a, which encodes an enzyme that regulates phosphatidylcholine synthesis.

Data table header descriptions
ID_REF
VALUE Expression Console RMA, Linear signal

Data table
ID_REF VALUE
17200001 100.4
17200003 100.2
17200005 40.0
17200007 45.4
17200009 102.8
17200011 38.7
17200013 53.0
17200015 73.8
17200017 17.3
17200019 24.1
17200021 46.8
17200023 131.5
17200025 66.1
17200027 130.9
17200029 58.0
17200031 27.6
17200033 34.3
17200035 23.7
17200037 70.7
17200039 8.0

Total number of rows: 41345

Table truncated, full table size 574 Kbytes.




Supplementary file Size Download File type/resource
GSM2991908_FedControl9.CEL.gz 9.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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