|
| Status |
Public on Dec 27, 2008 |
| Title |
Renal Clear Cell Carcinoma_TB3826 |
| Sample type |
RNA |
| |
|
| Source name |
mRNA from Renal Clear Cell Carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
VHL: Deficiant
HIF: HIF-2
Organism Part: Kidney
Disease State: Clear Cell Carcinoma
BioSourceType: Frozen Sample
Sex: Male
BioSource Provider: CHTN
Age: 60
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, with additional purification with Qiagen Rneasy columns
|
| Label |
biotin
|
| Label protocol |
Nugen Ovation version 2 was used for amplification, labeling and fragmentation
|
| |
|
| Hybridization protocol |
3.5 ug of cDNA was hybridized on the Affymetrix U133A V2.0 Chip following standard protocols.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip scanner 3000 7G
|
| Description |
Gene expression data from Human RCC sample Sample collection: Frozen RCCs were obtained through the Collaborative Human Tissue Network Sample quality control: H&E staining was performed on each sample, and they were confirmed to have clear cell morphology
|
| Data processing |
The data were extracted with Microarray Suite version 5.0 (MAS 5.0) and normalized with the GC-RMA algorithm in Array Assist Lite 3.4
|
| |
|
| Submission date |
Jun 26, 2008 |
| Last update date |
Oct 21, 2011 |
| Contact name |
Bradley Wubbenhorst |
| E-mail(s) |
bwubb@mail.med.upenn.edu
|
| Phone |
215-898-0248
|
| Organization name |
University of Pennsylvannia
|
| Street address |
421 Curie Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE11904 |
Gene expression analysis in primary human renal tumors, categorized by VHL genotype and HIF-alpha expression |
|
| Relations |
| Reanalyzed by |
GSE33093 |
