|
Status |
Public on Dec 27, 2008 |
Title |
Renal Clear Cell Carcinoma_TB4045 |
Sample type |
RNA |
|
|
Source name |
mRNA from Renal Clear Cell Carcinoma
|
Organism |
Homo sapiens |
Characteristics |
VHL: WT HIF: Negative Organism Part: Kidney Disease State: Clear Cell Carcinoma BioSourceType: Frozen Sample Sex: Male BioSource Provider: CHTN Age: Unknown
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, with additional purification with Qiagen Rneasy columns
|
Label |
biotin
|
Label protocol |
Nugen Ovation version 2 was used for amplification, labeling and fragmentation
|
|
|
Hybridization protocol |
3.5 ug of cDNA was hybridized on the Affymetrix U133A V2.0 Chip following standard protocols.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Genechip scanner 3000 7G
|
Description |
Gene expression data from Human RCC sample Sample collection: Frozen RCCs were obtained through the Collaborative Human Tissue Network Sample quality control: H&E staining was performed on each sample, and they were confirmed to have clear cell morphology
|
Data processing |
The data were extracted with Microarray Suite version 5.0 (MAS 5.0) and normalized with the GC-RMA algorithm in Array Assist Lite 3.4
|
|
|
Submission date |
Jun 26, 2008 |
Last update date |
Oct 21, 2011 |
Contact name |
Bradley Wubbenhorst |
E-mail(s) |
bwubb@mail.med.upenn.edu
|
Phone |
215-898-0248
|
Organization name |
University of Pennsylvannia
|
Street address |
421 Curie Blvd
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE11904 |
Gene expression analysis in primary human renal tumors, categorized by VHL genotype and HIF-alpha expression |
|
Relations |
Reanalyzed by |
GSE33093 |