|
| Status |
Public on Sep 30, 2009 |
| Title |
High TCF activity Huh7_S33Y |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
42126 |
| Tag Length |
10 |
| |
|
| Source name |
Huh7 stably expressing S33Y beta-catenin
|
| Organism |
Homo sapiens |
| Characteristics |
Huh7 liver cancer cell line
Stably expresses mutant(S33Y) beta-catenin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from early passages of tranfected cells cell lines (referred to as “Huh7-S33Y” and “Huh7-Vec” based on construct integrated into genome of Huh7) using the guanidine isothiocyanate method.
|
| |
|
| Description |
We performed SAGE experiments with Invitrogen's I-SAGE kit (Invitrogen, San Diego, CA USA) according to the manufacturer’s instructions and EMBO’s SAGE manual, by using 25 ug of total RNA from both samples. The kit manual was followed until preparation of 100 bp ditags. After this step, some minor modifications were made as described in the EMBO’s SAGE manual. Sequencing was performed using an Amersham 96 capillary automatic DNA sequencer.
|
| |
|
| Submission date |
Jun 27, 2008 |
| Last update date |
Jun 22, 2009 |
| Contact name |
Ersen Kavak |
| E-mail(s) |
ersenkavak@gmail.com
|
| Phone |
0046707875729
|
| URL |
http://aklab.boun.edu.tr
|
| Organization name |
Bogazici University
|
| Department |
Molecular Biology and Genetics
|
| Lab |
AKLAB (Ahmet Koman Lab)
|
| Street address |
Bogazici Uni. North Campus, North otopark building, 212
|
| City |
Istanbul |
| ZIP/Postal code |
34342 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL4 |
| Series (2) |
| GSE11912 |
Comparison of TCF hyper-activated and control Huh7 cells - SAGE |
| GSE11956 |
Comparison of TCF hyper-activated and control Huh7 cells |
|
