|
| Status |
Public on Nov 21, 2019 |
| Title |
Act5C-G4; dLsd1 RNAi_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
ovaries
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Act5C-G4; dLsd1 RNAi
gender: female
|
| Growth protocol |
Flies were raised at 25°C, 70% humidity on standard Drosophila medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from three independent preparations from Drosophila ovaries (2 days on yeast) using 500 µl Trizol (Invitrogen). RNA was treated with DNA-free™ DNA Removal Kit (Ambion) for 30 minutes at 37°C as described by the manufacturer
|
| Label |
cy3
|
| Label protocol |
The labeling was done using the Low Input Quick Amp Labeling kit (Agilent)
|
| |
|
| Hybridization protocol |
The slides (Drosophila Gene Expression Microarray, 4x44K ref 021791) were hybridized following the One-Color Microarray-Based Gene Expression Analysis Protocol (Agilent)
|
| Scan protocol |
The slides were scanned on a Tecan MS200 scanner.
|
| Description |
gene expression upon dLsd1 depletion by RNAi
|
| Data processing |
The median signal of each spot in the hybridized arrays was determined and quantified using Feature Extraction software v11.5.1.1. The data from all the microarrays were transformed in log2 intensity. Then they were normalized together using the “limma” package function “normalizeQuantiles” and the “quantile” method. Normalization of the data was performed using the Bioconductor packages (http://www.bioconductor.org) and R software v3.1.2.
|
| |
|
| Submission date |
Feb 16, 2018 |
| Last update date |
Nov 21, 2019 |
| Contact name |
Luisa Di Stefano |
| E-mail(s) |
luisa.di-stefano@univ-tlse3.fr
|
| Organization name |
LBCMCP, Centre de Biologie Intégrative (CBI)
|
| Street address |
118 Route de Narbonne
|
| City |
Toulouse |
| ZIP/Postal code |
31062 |
| Country |
France |
| |
|
| Platform ID |
GPL16820 |
| Series (1) |
| GSE110751 |
Genome-wide study of the role of the histone demethylase dLsd1 in oogenesis |
|
