|
| Status |
Public on May 12, 2009 |
| Title |
2 adipose tissue energy restriction rev rep |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Energy restriction-2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: female adipose tissue
|
| Biomaterial provider |
Third Faculty of Medicine of Charles University and at the Institute for Mother and Child Care in Prague, Czech Republic
|
| Treatment protocol |
Energy restriction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Needle microbiopsy of subcutaneous adipose tissue was performed under local anesthesia from the abdominal region. Total RNA were isolated from adipose tissue biopsies with Qiagen RNeasy kit , Quality and quantity were determined by capilary electrophoresis (Experion, BioRad laboratories)
|
| Label |
Cy5
|
| Label protocol |
Agilent low RNA input amplification kit
|
| |
|
| Channel 2 |
| Source name |
Basal-2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: female adipose tissue
|
| Biomaterial provider |
Third Faculty of Medicine of Charles University and at the Institute for Mother and Child Care in Prague, Czech Republic
|
| Treatment protocol |
Basal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Needle microbiopsy of subcutaneous adipose tissue was performed under local anesthesia from the abdominal region. Total RNA were isolated from adipose tissue biopsies with Qiagen RNeasy kit , Quality and quantity were determined by capilary electrophoresis (Experion, BioRad laboratories)
|
| Label |
Cy3
|
| Label protocol |
Agilent low RNA input amplification kit
|
| |
|
| |
| Hybridization protocol |
according to the manufacturer instructions (Agilent Technologies)
|
| Scan protocol |
Data acquisition from microarrays was obtained with a GenePix 4000B scanner (Axon instruments) and image processing was performed with Feature Extraction 8.5 (Agilent Technologies)
|
| Description |
obese premenopausal women followed a dietary intervention program.
|
| Data processing |
log transformation and Lowess normalization without background substraction
|
| |
|
| Submission date |
Jul 02, 2008 |
| Last update date |
Mar 12, 2010 |
| Contact name |
Frederic Capel |
| E-mail(s) |
frederic.capel@free.fr
|
| Organization name |
INSERM
|
| Department |
INSERM UPS U858
|
| Lab |
Laboratoire de recherche sur les obésités
|
| Street address |
1 Av Jean Poulhes
|
| City |
Toulouse |
| ZIP/Postal code |
31432 |
| Country |
France |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE11975 |
Gene Expression in Adipose Tissue during Weight Loss |
|
