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Sample GSM302963 Query DataSets for GSM302963
Status Public on May 12, 2009
Title 1 adipose tissue weight stabilization
Sample type RNA
 
Channel 1
Source name Weight stabilization-1
Organism Homo sapiens
Characteristics tissue: female adipose tissue
Biomaterial provider Third Faculty of Medicine of Charles University and at the Institute for Mother and Child Care in Prague, Czech Republic
Treatment protocol Weight stabilization
Extracted molecule total RNA
Extraction protocol Needle microbiopsy of subcutaneous adipose tissue was performed under local anesthesia from the abdominal region. Total RNA were isolated from adipose tissue biopsies with Qiagen RNeasy kit , Quality and quantity were determined by capilary electrophoresis (Experion, BioRad laboratories)
Label Cy5
Label protocol Agilent low RNA input amplification kit
 
Channel 2
Source name Energy restriction-1
Organism Homo sapiens
Characteristics tissue: female adipose tissue
Biomaterial provider Third Faculty of Medicine of Charles University and at the Institute for Mother and Child Care in Prague, Czech Republic
Treatment protocol Energy restriction
Extracted molecule total RNA
Extraction protocol Needle microbiopsy of subcutaneous adipose tissue was performed under local anesthesia from the abdominal region. Total RNA were isolated from adipose tissue biopsies with Qiagen RNeasy kit , Quality and quantity were determined by capilary electrophoresis (Experion, BioRad laboratories)
Label Cy3
Label protocol Agilent low RNA input amplification kit
 
 
Hybridization protocol according to the manufacturer instructions (Agilent Technologies)
Scan protocol Data acquisition from microarrays was obtained with a GenePix 4000B scanner (Axon instruments) and image processing was performed with Feature Extraction 8.5 (Agilent Technologies)
Description obese premenopausal women followed a dietary intervention program.
Data processing log transformation and Lowess normalization without background substraction
 
Submission date Jul 02, 2008
Last update date Mar 12, 2010
Contact name Frederic Capel
E-mail(s) frederic.capel@free.fr
Organization name INSERM
Department INSERM UPS U858
Lab Laboratoire de recherche sur les obésités
Street address 1 Av Jean Poulhes
City Toulouse
ZIP/Postal code 31432
Country France
 
Platform ID GPL1708
Series (1)
GSE11975 Gene Expression in Adipose Tissue during Weight Loss

Data table header descriptions
ID_REF
VALUE Normalized log ratio (channel 1/channel 2)

Data table
ID_REF VALUE
1 0.311946264
2 0.034629331
3 0.230087891
4 0.273830517
5 0.41802025
6 0.122030995
7 0.156099112
8 -0.02416145
9 0.398954475
10 0.44144077
11 0.728763554
12 0.293247751
13 0.586870251
14 0.180940254
15 0.514508911
16 0.255329347
17 0.20728009
18 -0.004289073
19 0.475232916
20 0.286874274

Total number of rows: 43931

Table truncated, full table size 777 Kbytes.




Supplementary file Size Download File type/resource
GSM302963.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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