|
| Status |
Public on Jul 30, 2008 |
| Title |
HeLa cells transfected with miR-181 duplex versus control transfected HeLa, 24 hours |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mock transfected HeLa cells, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Expression profile of mRNAs from mock-transfected HeLa cells for 24 hours
|
| Treatment protocol |
Cells grown in SILAC media containing heavy amino acids (Arg and Lys) were mock-transfected with lipofectamine 2000 (Invitrogen).
|
| Growth protocol |
HeLa cells were grown in SILAC DMEM media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus
|
| Label |
Cy3
|
| Label protocol |
Agilent 1 Cycle Labelling Standard Protocol
|
| |
|
| Channel 2 |
| Source name |
HeLa cells transfected with miR-181 duplex, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Expression profile of mRNAs from HeLa cells transfected with miR-181 duplex for 24 hours
|
| Treatment protocol |
Cells grown in SILAC media containing light amino acids (Arg and Lys) were transfected with miRNA-181 duplexes.
|
| Growth protocol |
HeLa cells were grown in SILAC DMEM media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus
|
| Label |
Cy5
|
| Label protocol |
Agilent 1 Cycle Labelling Standard Protocol
|
| |
|
| |
| Hybridization protocol |
Agilent Sure Hybe Protocol
|
| Scan protocol |
Agilent Scanner
|
| Description |
Expression profiling comparison of mRNAs in miRNA-181-transfected HeLa cells vs. mock-transfected HeLa cells
|
| Data processing |
Agilent Feature Extraction Software, Protocol: GE2-v5_95_Feb07
|
| |
|
| Submission date |
Jul 02, 2008 |
| Last update date |
Jul 15, 2008 |
| Contact name |
Whitehead Institute |
| E-mail(s) |
sgupta@wi.mit.edu
|
| Phone |
617-324-0339
|
| Organization name |
Whitehead Institute
|
| Department |
Genome Technology Core
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE11968 |
Analysis of HeLa cells after transfection with miR-1, miR-124 and miR-181. |
| GSE12075 |
The impact of microRNAs on protein output |
|
