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Sample GSM303237 Query DataSets for GSM303237
Status Public on Jul 30, 2008
Title Neutrophils cultured from miR-223 null mouse bone marrow - Rep 1
Sample type RNA
 
Source name Neutrophils
Organism Mus musculus
Characteristics mRNA profiling of neutrophils in vitro cultured from miR-223 null mouse bone marrow - Rep 1
Treatment protocol Live neutrophils were isolated using MACS.
Growth protocol BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 6 days and subsequent culture for 42 hours without SCF. During the time, maturation to neutrophils occurs.
Extracted molecule total RNA
Extraction protocol Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
Label Biotin
Label protocol Affy Standard labeling Protocol
 
Hybridization protocol Affy Standard Hyb Protocol
Scan protocol Affy Scanner
Description mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils
Data processing GCOS and RMAExpress software
 
Submission date Jul 02, 2008
Last update date Aug 28, 2018
Contact name Whitehead Institute
E-mail(s) sgupta@wi.mit.edu
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL1261
Series (2)
GSE11973 Wild-type cultured neutrophils versus miR-223 null cultured neutrophils
GSE12075 The impact of microRNAs on protein output
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal Intenstiy (Log2)

Data table
ID_REF VALUE
1415670_AT 8.864704
1415671_AT 11.355082
1415672_AT 11.268411
1415673_AT 9.148849
1415674_A_AT 10.311984
1415675_AT 9.540609
1415676_A_AT 11.892856
1415677_AT 10.409448
1415678_AT 10.397074
1415679_AT 12.110623
1415680_AT 8.721903
1415681_AT 10.364969
1415682_AT 7.570303
1415683_AT 11.096360
1415684_AT 9.673695
1415685_AT 8.988747
1415686_AT 9.218534
1415687_A_AT 13.183028
1415688_AT 9.927662
1415689_S_AT 7.950511

Total number of rows: 45101

Table truncated, full table size 902 Kbytes.




Supplementary file Size Download File type/resource
GSM303237.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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