|
Status |
Public on Jul 30, 2008 |
Title |
4 days cultures from wild-type mouse bone marrow progenitors - Rep 1 |
Sample type |
RNA |
|
|
Source name |
4 days cultured BM progenitors from wild-type mouse bone marrow
|
Organism |
Mus musculus |
Characteristics |
mRNA profiling of 4 days cultures from wild-type mouse bone marrow progenitors - Rep 1
|
Treatment protocol |
Cells were sorted for ckit-PE positive and Gr-1-APC negative population using FACSAria (BD Biosciences).
|
Growth protocol |
BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 4 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
|
Label |
Biotin
|
Label protocol |
Affy Standard Labeling Protocol
|
|
|
Hybridization protocol |
Affy Standard Hyb Protocol
|
Scan protocol |
Affy Scan Protocol
|
Description |
mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils during development of BM progenitors to mature neutrophils. This data shows expression profile of mRNAs from 4 days cultured progenitors from wild-type mouse bone marrow.
|
Data processing |
GCOS and RMAExpress software
|
|
|
Submission date |
Jul 03, 2008 |
Last update date |
Aug 28, 2018 |
Contact name |
Whitehead Institute |
E-mail(s) |
sgupta@wi.mit.edu
|
Phone |
617-324-0339
|
Organization name |
Whitehead Institute
|
Department |
Genome Technology Core
|
Street address |
9 Cambridge Center
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02141 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE12003 |
4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils |
GSE12075 |
The impact of microRNAs on protein output |
|
Relations |
Reanalyzed by |
GSE119085 |