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Sample GSM303495 Query DataSets for GSM303495
Status Public on Jul 30, 2008
Title 4 days cultures from miR-223 null mouse bone marrow progenitors - Rep 1
Sample type RNA
 
Source name 4 days cultured BM progenitors from miR-223 null mouse bone marrow
Organism Mus musculus
Characteristics mRNA profiling of 4 days cultures from miR-223 null mouse bone marrow progenitors - Rep 1
Treatment protocol Cells were sorted for ckit-PE positive and Gr-1-APC negative population using FACSAria (BD Biosciences).
Growth protocol BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 4 days.
Extracted molecule total RNA
Extraction protocol Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
Label Biotin
Label protocol Affy Standard Labeling Protocol
 
Hybridization protocol Affy Standard Hyb Protocol
Scan protocol Affy Scan Protocol
Description mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils during development of BM progenitors to mature neutrophils. This data shows expression profile of mRNAs from 4 days cultured progenitors from miR-223 null mouse bone marrow.
Data processing GCOS and RMAExpress software
 
Submission date Jul 03, 2008
Last update date Aug 28, 2018
Contact name Whitehead Institute
E-mail(s) sgupta@wi.mit.edu
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL1261
Series (2)
GSE12003 4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils
GSE12075 The impact of microRNAs on protein output
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal Intensity (Log2)

Data table
ID_REF VALUE
1415670_AT 9.889081
1415671_AT 11.255327
1415672_AT 11.348035
1415673_AT 10.771189
1415674_A_AT 10.130442
1415675_AT 9.594633
1415676_A_AT 12.104434
1415677_AT 9.118778
1415678_AT 10.081198
1415679_AT 11.764560
1415680_AT 10.438824
1415681_AT 10.710661
1415682_AT 8.607322
1415683_AT 11.438702
1415684_AT 8.877566
1415685_AT 9.334173
1415686_AT 9.556262
1415687_A_AT 11.394907
1415688_AT 10.631303
1415689_S_AT 8.571744

Total number of rows: 45101

Table truncated, full table size 902 Kbytes.




Supplementary file Size Download File type/resource
GSM303495.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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