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Sample GSM303497 Query DataSets for GSM303497
Status Public on Jul 30, 2008
Title 8 days cultures from wild-type mouse bone marrow progenitors - Rep 1
Sample type RNA
 
Source name 8 day cultured and sorted mature neutrophils
Organism Mus musculus
Characteristics Expression profile of mRNAs of 8 days cultured and sorted mature neutrophls from wild-type mouse bone marrow progenitors - Rep 1
Treatment protocol Cells were sorted for ckit-PE negative and Gr-1-APC positive population using FACSAria (BD Biosciences).
Growth protocol BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 6 days and subsequent culture without SCF for 42 hours.
Extracted molecule total RNA
Extraction protocol Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
Label Biotin
Label protocol Affy Standard Labeling Protocol
 
Hybridization protocol Affy Standard Hyb Protocol
Scan protocol Affy Scan Protocol
Description mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils during development of BM progenitors to mature neutrophils. This data shows expression profile of mRNAs from 8 days cultured and sorted mature neutrophils from wild-type mouse bone marrow.
Data processing GCOS and RMAExpress software
 
Submission date Jul 03, 2008
Last update date Aug 28, 2018
Contact name Whitehead Institute
E-mail(s) sgupta@wi.mit.edu
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL1261
Series (2)
GSE12003 4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils
GSE12075 The impact of microRNAs on protein output
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal Intensity (Log2)

Data table
ID_REF VALUE
1415670_AT 9.011919
1415671_AT 11.764469
1415672_AT 11.106338
1415673_AT 7.352361
1415674_A_AT 10.043955
1415675_AT 9.178175
1415676_A_AT 11.278594
1415677_AT 11.031226
1415678_AT 10.760014
1415679_AT 12.084722
1415680_AT 9.109142
1415681_AT 10.058769
1415682_AT 7.742371
1415683_AT 11.273192
1415684_AT 9.361398
1415685_AT 7.965687
1415686_AT 9.693543
1415687_A_AT 13.148552
1415688_AT 9.987626
1415689_S_AT 8.394988

Total number of rows: 45101

Table truncated, full table size 902 Kbytes.




Supplementary file Size Download File type/resource
GSM303497.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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