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Sample GSM303501 Query DataSets for GSM303501
Status Public on Jul 30, 2008
Title 4 days cultures from miR-223 null mouse bone marrow progenitors - Rep 2
Sample type RNA
 
Source name 4 days cultured BM progenitors from miR-223 null mouse
Organism Mus musculus
Characteristics mRNA profiling of 4 days cultures from miR-223 null mouse bone marrow progenitors - Rep 2
Treatment protocol Cells were sorted for ckit-PE positive and Gr-1-APC negative population using FACSAria (BD Biosciences).
Growth protocol BM-progenitors were cultured in SILAC-IMDM with G-CSF and SCF for 4 days.
Extracted molecule total RNA
Extraction protocol Tri Reagent (Ambion) followed by RNeasy Plus (Qiagen)
Label Biotin
Label protocol Affy Standard Labeling Protocol
 
Hybridization protocol Affy Standard Hyb Protocol
Scan protocol Affy Scan Protocol
Description mRNA expression profile comparison between wild-type cultured murine neutrophils versus miR-223 null cultured murine neutrophils during development of BM progenitors to mature neutrophils. This data shows expression profile of mRNAs from 4 days cultured progenitors from miR-223 null mouse bone marrow.
Data processing GCOS and RMAExpress software
 
Submission date Jul 03, 2008
Last update date Aug 28, 2018
Contact name Whitehead Institute
E-mail(s) sgupta@wi.mit.edu
Phone 617-324-0339
Organization name Whitehead Institute
Department Genome Technology Core
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02141
Country USA
 
Platform ID GPL1261
Series (2)
GSE12003 4 days cultured progenitors and 8 days cultured mature neutrophils from WT vs miR-223 null neutrophils
GSE12075 The impact of microRNAs on protein output
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal Intensity (Log2)

Data table
ID_REF VALUE
1415670_AT 9.854607
1415671_AT 11.283952
1415672_AT 11.359027
1415673_AT 10.842671
1415674_A_AT 10.122091
1415675_AT 9.575099
1415676_A_AT 12.092137
1415677_AT 9.228344
1415678_AT 10.045629
1415679_AT 11.717798
1415680_AT 10.343417
1415681_AT 10.590671
1415682_AT 8.558041
1415683_AT 11.396890
1415684_AT 8.853692
1415685_AT 9.439826
1415686_AT 9.405993
1415687_A_AT 11.426040
1415688_AT 10.542215
1415689_S_AT 8.553540

Total number of rows: 45101

Table truncated, full table size 902 Kbytes.




Supplementary file Size Download File type/resource
GSM303501.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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