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Sample GSM3039417 Query DataSets for GSM3039417
Status Public on Mar 14, 2018
Title Testis from Prss54KO mouse_rep3
Sample type RNA
 
Source name Testis_Prss54KO mouse
Organism Mus musculus
Characteristics strain background: mixed 129Sv/C57BL/6
genotype/variation: Prss54KO
gender: male
age: 6-8 weeks
tissue: testis
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer's recommendations and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies, Santa Clara, CA, US) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).
 
Hybridization protocol Each slide was hybridized with 1.65ug Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions.
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=5μm, PMT 100%, 10%, 16bit.
Description Prss54ko3L testis
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, GeneSpring Software 12.6.1 (Agilent technologies, Santa Clara, CA, US).
 
Submission date Mar 13, 2018
Last update date Mar 15, 2018
Contact name Xuan Shang
E-mail(s) 15000081962@163.com
Phone 15000081962
Organization name Shanghai Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Department Research Center for Experimental Medicine
Street address No.197, Ruijin 2nd Road, Huangpu District, Shanghai
City shanghai
State/province shanghai
ZIP/Postal code 200025
Country China
 
Platform ID GPL7202
Series (1)
GSE111758 Identification differentially expressed genes in the mouse testis caused by Prss54 deficiency

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 14.661253
DarkCorner 3.0366275
A_52_P616356 2.9606826
A_52_P580582 6.999807
A_52_P403405 15.469091
A_52_P819156 8.387951
A_51_P331831 6.974041
A_51_P430630 2.9524426
A_52_P502357 2.9515324
A_52_P299964 2.950762
A_51_P356389 3.6550026
A_52_P684402 12.989548
A_51_P414208 8.6475315
A_51_P280918 10.458692
A_52_P613688 6.5658116
A_52_P258194 12.046443
A_52_P229271 7.0222597
A_52_P214630 9.196889
A_52_P579519 15.442321
A_52_P979997 2.9543266

Total number of rows: 41267

Table truncated, full table size 907 Kbytes.




Supplementary file Size Download File type/resource
GSM3039417_Prss54_3_L_251486840910_S01_GE1_107_Sep09_1_4.txt.gz 9.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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