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Sample GSM304308 Query DataSets for GSM304308
Status Public on Aug 26, 2008
Title Expression profile 24 hours post infection for R2G e1a mutant
Sample type RNA
 
Channel 1
Source name infected IMR90 fibroblasts
Organism Homo sapiens
Characteristics infected IMR90 female fibroblasts total labeled RNA
Extracted molecule total RNA
Extraction protocol qiagen RNAeasy Kit for total RNA extraction. Sample has been extracted at 24 hours post infection
Label cy5
Label protocol Two-Color Microarray-Based Gene Expression (Quick Amp Labeling) Protocol G4140-90050 v.5.7
 
Channel 2
Source name Mock infected IMR90 fibroblasts
Organism Homo sapiens
Characteristics Mock infected IMR90 female fibroblasts
Extracted molecule total RNA
Extraction protocol qiagen RNAeasy Kit for total RNA extraction. Sample has been extracted at 24 hours post infection
Label cy3
Label protocol Two-Color Microarray-Based Gene Expression (Quick Amp Labeling) Protocol G4140-90050 v.5.7
 
 
Hybridization protocol Two-Color Microarray-Based Gene Expression (Quick Amp Labeling) Protocol G4140-90050 v.5.7
Scan protocol Agilent DNA Microarray Scanner
Description none
Data processing Triplicate samples of 250 ng of RNA from three independent infections from dl1500- and mock-infected primary IMR90 fibroblasts cells were amplified and labeled with Cy5 and Cy3 (Perkin Elmer), respectively, using the Agilent Two-Color Microarray-Based Gene Expression (Quick Amp Labeling) Protocol according to manufacturer's instructions. Labeled RNA was hybridized to the Agilent Human whole-genome array (G2534-60011) and Expression data were extracted using Agilent Feature Extraction software (version 9.1.3.1). Raw data was natural log (ln) transformed and signals from multiple probes for the same gene were averaged. Each array was normalized so that the mean was zero and standard deviation was one. Data from three independent replicate experiments were averaged.
 
Submission date Jul 08, 2008
Last update date Aug 25, 2008
Contact name Siavash K Kurdistani
E-mail(s) Skurdistani@mednet.ucla.edu
Organization name UCLA
Department Biological Chemistry
Lab Kurdistani
Street address 615 Charles E Young Dr South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL7031
Series (2)
GSE12046 Expression profiles and ChIP on chip genomewide experiments with R2G mutant virus
GSE12543 Profiles and ChIP on chip experiments with dl1500 virus (expressing WT small e1a), R2G and deltaCR2 mutant viruses

Data table header descriptions
ID_REF
VALUE natural Log of ratio

Data table
ID_REF VALUE
1 -0.118435
2 0.779876
3 -0.386117
4 -0.373248
5 0.0873
6 -0.037844
7 0.093747
8 -0.027287
9 0.267119
10 0.360165
11 -0.017614
12 -0.903818
13 0.011827
14 -0.119246
15 0.023766
16 0.7679
17 0.503678
18 0.552947
19 -0.143894
20 0

Total number of rows: 13792

Table truncated, full table size 183 Kbytes.




Supplementary file Size Download File type/resource
GSM304308_nl_251485011757_S01_GE2-v5_91_0806_1_1.txt.gz 12.8 Mb (ftp)(http) TXT
GSM304308_nl_251485011757_S01_GE2-v5_91_0806_1_2.txt.gz 12.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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