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Sample GSM304386 Query DataSets for GSM304386
Status Public on Oct 02, 2009
Title blood_nonresponder_patient2
Sample type RNA
Source name Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
Organism Homo sapiens
Characteristics disease state: nonresponder
gender: female
tissue: whole blood
treatment stage: baseline (prior infliximab infusion)
Treatment protocol Paxgene tube conservation at -80ºC
Extracted molecule total RNA
Extraction protocol Whole blood RNA was extracted from whole blood preserved in Paxgene tubes using the PAXgene RNA Isolation kit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
Hybridization protocol Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol Scanning was performed on the Illumina(r) BeadStation 500x.
Description Whole blood RNA, Infliximab non-responder
Data processing Log2 transformation of BeadStudio raw data, gene filtering of Beadchip version2 RefSeq valid probes, quantile normalization using R.
Submission date Jul 09, 2008
Last update date Mar 29, 2010
Contact name Antonio Julià
Phone +34 934029082
Organization name Vall d'Hebron Hospital Research Institute
Department Rheumatology Research Group
Lab Rheumatology Research Group
Street address c/ Baldiri Reixac nº15
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
Platform ID GPL2507
Series (1)
GSE12051 Microarray predictor of response to infliximab in rheumatoid arthritis (RA) patients

Data table header descriptions
VALUE Quantile normalized signal intensity

Data table
GI_10047089-S 6.4709019132588
GI_10047091-S 6.73507093096967
GI_10047093-S 6.81712363338828
GI_10047099-S 6.42264488439549
GI_10047103-S 9.1443963697267
GI_10047123-S 7.02256370082636
GI_10047133-I 6.59678451479955
GI_10092578-S 6.50919718660349
GI_10092585-S 6.81926676919727
GI_10092596-S 7.76747251989611
GI_10092600-S 7.01423248402962
GI_10092602-S 6.55930646072333
GI_10092611-A 7.75831481546987
GI_10092616-S 6.77828043413452
GI_10092618-S 9.47354359090237
GI_10092638-S 6.52103263208945
GI_10092658-S 6.65789831062314
GI_10092668-S 6.51117691117906
GI_10092672-S 7.2854787892006
GI_10190657-S 6.65789831062314

Total number of rows: 17454

Table truncated, full table size 521 Kbytes.

Supplementary file Size Download File type/resource
GSM304386.txt.gz 219.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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