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Sample GSM304402 Query DataSets for GSM304402
Status Public on Oct 02, 2009
Title blood_responder_patient7
Sample type RNA
 
Source name Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
Organism Homo sapiens
Characteristics disease state: responder
gender: female
tissue: whole blood
treatment stage: baseline (prior infliximab infusion)
Treatment protocol Paxgene tube conservation at -80ºC
Extracted molecule total RNA
Extraction protocol Whole blood RNA was extracted from whole blood preserved in Paxgene tubes using the PAXgene RNA Isolation kit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
 
Hybridization protocol Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol Scanning was performed on the Illumina(r) BeadStation 500x.
Description Whole blood RNA, Infliximab responder
Data processing Log2 transformation of BeadStudio raw data, gene filtering of Beadchip version2 RefSeq valid probes, quantile normalization using R.
 
Submission date Jul 09, 2008
Last update date Mar 29, 2010
Contact name Antonio Julià
E-mail(s) toni.julia@vhir.org
Phone +34 934029082
Organization name Vall d'Hebron Hospital Research Institute
Department Rheumatology Research Group
Lab Rheumatology Research Group
Street address c/ Baldiri Reixac nº15
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
 
Platform ID GPL2507
Series (1)
GSE12051 Microarray predictor of response to infliximab in rheumatoid arthritis (RA) patients

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
GI_10047089-S 6.63632060978695
GI_10047091-S 6.51828946210273
GI_10047093-S 6.74960299989235
GI_10047099-S 6.50293303668137
GI_10047103-S 8.47669674447642
GI_10047123-S 6.7359755539384
GI_10047133-I 6.65109967337502
GI_10092578-S 6.43302568779534
GI_10092585-S 6.94412892448496
GI_10092596-S 8.23455131952263
GI_10092600-S 6.70113315303884
GI_10092602-S 6.46096764712645
GI_10092611-A 8.07292141061298
GI_10092616-S 6.78386343180967
GI_10092618-S 9.48330887576958
GI_10092638-S 6.47692992926902
GI_10092658-S 6.39496661546066
GI_10092668-S 6.77832984065099
GI_10092672-S 7.1908322371993
GI_10190657-S 6.60553306098495

Total number of rows: 17454

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM304402.txt.gz 212.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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