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Sample GSM304413 Query DataSets for GSM304413
Status Public on Oct 02, 2009
Title blood_responder_patient12
Sample type RNA
 
Source name Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
Organism Homo sapiens
Characteristics disease state: responder
gender: female
tissue: whole blood
treatment stage: baseline (prior infliximab infusion)
Treatment protocol Paxgene tube conservation at -80ºC
Extracted molecule total RNA
Extraction protocol Whole blood RNA was extracted from whole blood preserved in Paxgene tubes using the PAXgene RNA Isolation kit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
 
Hybridization protocol Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol Scanning was performed on the Illumina(r) BeadStation 500x.
Description Whole blood RNA, Infliximab responder
Data processing Log2 transformation of BeadStudio raw data, gene filtering of Beadchip version2 RefSeq valid probes, quantile normalization using R.
 
Submission date Jul 09, 2008
Last update date Mar 29, 2010
Contact name Antonio Julià
E-mail(s) toni.julia@vhir.org
Phone +34 934029082
Organization name Vall d'Hebron Hospital Research Institute
Department Rheumatology Research Group
Lab Rheumatology Research Group
Street address c/ Baldiri Reixac nº15
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
 
Platform ID GPL2507
Series (1)
GSE12051 Microarray predictor of response to infliximab in rheumatoid arthritis (RA) patients

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
GI_10047089-S 6.44882414379852
GI_10047091-S 6.49779619884559
GI_10047093-S 6.62364457292112
GI_10047099-S 6.72238768469893
GI_10047103-S 8.44480453103837
GI_10047123-S 7.27732740503036
GI_10047133-I 6.53652085614012
GI_10092578-S 6.55764587979197
GI_10092585-S 6.7760762124381
GI_10092596-S 7.88231832176278
GI_10092600-S 7.1295926654541
GI_10092602-S 6.56713523590095
GI_10092611-A 8.14310757051892
GI_10092616-S 6.6875487345509
GI_10092618-S 9.49404822596399
GI_10092638-S 6.6170757535582
GI_10092658-S 6.47555338448147
GI_10092668-S 6.45139996716571
GI_10092672-S 7.12768587859972
GI_10190657-S 6.56898182167991

Total number of rows: 17454

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM304413.txt.gz 215.6 Kb (ftp)(http) TXT
Processed data included within Sample table

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