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Sample GSM304414 Query DataSets for GSM304414
Status Public on Oct 02, 2009
Title blood_responder_patient13
Sample type RNA
Source name Rheumatoid arthritis patient, whole blood, at 8:30-9:30 morning
Organism Homo sapiens
Characteristics disease state: responder
gender: female
tissue: whole blood
treatment stage: baseline (prior infliximab infusion)
Treatment protocol Paxgene tube conservation at -80ºC
Extracted molecule total RNA
Extraction protocol Whole blood RNA was extracted from whole blood preserved in Paxgene tubes using the PAXgene RNA Isolation kit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
Hybridization protocol Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol Scanning was performed on the Illumina(r) BeadStation 500x.
Description Whole blood RNA, Infliximab responder
Data processing Log2 transformation of BeadStudio raw data, gene filtering of Beadchip version2 RefSeq valid probes, quantile normalization using R.
Submission date Jul 09, 2008
Last update date Mar 29, 2010
Contact name Antonio Julià
Phone +34 934029082
Organization name Vall d'Hebron Hospital Research Institute
Department Rheumatology Research Group
Lab Rheumatology Research Group
Street address c/ Baldiri Reixac nº15
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
Platform ID GPL2507
Series (1)
GSE12051 Microarray predictor of response to infliximab in rheumatoid arthritis (RA) patients

Data table header descriptions
VALUE Quantile normalized signal intensity

Data table
GI_10047089-S 6.63306244670066
GI_10047091-S 6.37615680521643
GI_10047093-S 6.8819356661959
GI_10047099-S 6.63930606740167
GI_10047103-S 8.61394927545108
GI_10047123-S 7.03410137696886
GI_10047133-I 6.5145245217724
GI_10092578-S 6.32675520904433
GI_10092585-S 6.60835125136729
GI_10092596-S 7.79035529997234
GI_10092600-S 7.19202330421108
GI_10092602-S 6.30036222375039
GI_10092611-A 8.12425028931844
GI_10092616-S 6.70412795034804
GI_10092618-S 9.68531920744813
GI_10092638-S 6.54394581340946
GI_10092658-S 6.48878343113229
GI_10092668-S 6.45157423034103
GI_10092672-S 7.27947535555361
GI_10190657-S 6.4680466167835

Total number of rows: 17454

Table truncated, full table size 522 Kbytes.

Supplementary file Size Download File type/resource
GSM304414.txt.gz 220.4 Kb (ftp)(http) TXT
Processed data included within Sample table

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