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Sample GSM3044865 Query DataSets for GSM3044865
Status Public on Apr 09, 2018
Title C_R3
Sample type SRA
 
Source name Liver
Organism Gallus gallus
Characteristics group: control
liver phenotype: non-fatty liver
Sex: male
age: 36 week
Extracted molecule total RNA
Extraction protocol Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit v2 was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing The sequenced data was filtered with the Sickle (https://github.com/najoshi/sickle) and SeqPrep (https://github.com/jstjohn/SeqPrep) software to remove the noise and low-quality sequences.
The clean reads were mapped on the reference genome of Gallus_gallus_5.0.
The expression abundance of the unigenes was calculated according to fragments per kilobase of exon per million (FPKM) fragments mapped.
Differentially expressed genes (DEGs), with a false discovery rate (FDR) < 0.05, were selected as the criteria for significant differences.
Genome_build: GCA_000002315.3
Supplementary_files_format_and_content: FPKM
 
Submission date Mar 15, 2018
Last update date Apr 09, 2018
Contact name yonghong zhang
E-mail(s) yonghong@jlu.edu.cn
Organization name Jilin University
Street address No.2699 Qianjin Street
City Changchun
State/province Jilin Province
ZIP/Postal code 130012
Country China
 
Platform ID GPL24517
Series (1)
GSE111909 Acquired paternal inheritance fatty liver transcriptomes induced by high-fat diet
Relations
BioSample SAMN08719877
SRA SRX3800308

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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