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Sample GSM3044866

Query DataSets for GSM3044866

Status

Public on Apr 09, 2018

Title

FL_R1

Sample type

SRA

Source name

Liver

Organism

Gallus gallus

Characteristics

group: paternal inheritance
liver phenotype: fatty liver
Sex: male
age: 36 week

Extracted molecule

total RNA

Extraction protocol

Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit v2 was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

HiSeq X Ten

Data processing

The sequenced data was filtered with the Sickle (https://github.com/najoshi/sickle) and SeqPrep (https://github.com/jstjohn/SeqPrep) software to remove the noise and low-quality sequences.
The clean reads were mapped on the reference genome of Gallus_gallus_5.0.
The expression abundance of the unigenes was calculated according to fragments per kilobase of exon per million (FPKM) fragments mapped.
Differentially expressed genes (DEGs), with a false discovery rate (FDR) < 0.05, were selected as the criteria for significant differences.
Genome_build: GCA_000002315.3
Supplementary_files_format_and_content: FPKM

Submission date

Mar 15, 2018

Last update date

Apr 09, 2018

Contact name

yonghong zhang

E-mail(s)

yonghong@jlu.edu.cn

Organization name

Jilin University

Street address

No.2699 Qianjin Street