|
| Status |
Public on Jul 18, 2008 |
| Title |
ES LUC esiRNA day4 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells
|
| Organism |
Mus musculus |
| Characteristics |
Strain: Oct4-Gip
Passage: 25
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by using the RNeasy Mini kit (Qiagen) and treated with DNase (Qiagen).
|
| Label |
biotin
|
| Label protocol |
One-cycle target labeling and control reagent package (Affymetrix)
|
| |
|
| Hybridization protocol |
15 µg fragmented cRNA was hybridised for 16 h at 45 °C to Mouse Genome 430 2.0 arrays which carry probes representing 45101 probe sets. Following hybridisation arrays were washed and stained with streptavidin-phycoerythrin in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
scanned using the Hewlett-Packard GeneChip Scanner 3000 7G.
|
| Description |
lipofection mediated esiRNA knockdown
|
| Data processing |
The image data were analysed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalisation method. After RMA normalisation (Irizarry et al., 2003) and outlier removement using the Nalimov test (p< 0.001) have been performed.
|
| |
|
| Submission date |
Jul 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Li Ding |
| E-mail(s) |
ding@mpi-cbg.de
|
| Organization name |
MPI-CBG
|
| Lab |
Buchholz Lab
|
| Street address |
Pfotenhauerstr. 108
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE12078 |
Ctr9 knockdown in mouse ES cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
