|
| Status |
Public on Jul 21, 2008 |
| Title |
C26_IP_3475_NOC |
| Sample type |
genomic |
| |
|
| Source name |
ChIP DNA from yeast Saccharomyces cerevisiae cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Saccharomyces cerevisiae (W303 background)
Genotype: MATa Brn1-9PK B box (386,600 chr V)
2 hours nocodazole arrested cells
Strain: Y3475
Antibody: anti-PK
|
| Treatment protocol |
Extraction was performed as previously described (Katou et al. 2003)
|
| Growth protocol |
Cells in YP + 2% glucose were synchronized in metaphase with the spindle depolymerising drug nocodazole
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction was performed as previously described (Katou et al. 2003).
|
| Label |
biotin
|
| Label protocol |
DNA was labeled with biotin-N6-ddATP using terminal transferase TdT (Roche, 400U/µl).
|
| |
|
| Hybridization protocol |
Hybridization was performed as previously described (Katou et al. 2003). Samples were hybridized for 16 hours at 42ºC in a hybridization oven (GeneChip Hybridization Oven 640, Affymetrix)
|
| Scan protocol |
The washing and scanning protocol, provided by Affymetrix, was performed automatically on a fluidics station (GeneChip fluidics station 450, Affymetrix)
|
| Description |
C26_IP_3475_NOC.CEL
Note: The header of C26_IP_3475_NOC.CEL incorrectly identifies the array as S_cerevisiaeR_4x. The correct array assignment is S_cerevisiaeF_4x.
|
| Data processing |
Data were processed following the protocol provided by Affymetrix (GCOS ver 1.4)
|
| |
|
| Submission date |
Jul 17, 2008 |
| Last update date |
Sep 04, 2008 |
| Contact name |
claudio d'ambrosio |
| E-mail(s) |
claudio.ambrosio@cancer.org.uk
|
| Organization name |
Cancer Research UK
|
| Street address |
44 Lincoln's Inn Fields
|
| City |
London |
| ZIP/Postal code |
WC2A 3PX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL7249 |
| Series (1) |
| GSE12149 |
Chip-chip of condensin subunits Brn1 and Smc4 and Scc2/4 and cohesin and Tfc3 (TFIIIC) on budding yeast chromosomes |
|
