|
| Status |
Public on Jul 30, 2008 |
| Title |
22027 |
| Sample type |
RNA |
| |
|
| Source name |
Ovarian Tumour, Serous LMP
|
| Organism |
Homo sapiens |
| Characteristics |
LMP
mutant KRAS
Grade Low
Stage IIA
Micropapillary feature Negative
(LMP) implants Non-invasive
Co-exhistant histology NA
LMP Cluster
PRIMARY
arrayed site Ovary
RNA taken from whole tumour sections
|
| Treatment protocol |
Frozen tissue stored at -80oC taken after surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and column chromotogrpahy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Affymetrix HG-U133 2.0plus expression array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GS300-7G scanner
|
| Description |
Gene expression data from human ovarian tumours
|
| Data processing |
The data were analyzed with Gene Chip Operating Software Version 1.4 using Affymetrix default analysis settings. The CEL files generated was later normalized using the RMA method
|
| |
|
| Submission date |
Jul 18, 2008 |
| Last update date |
Jul 30, 2008 |
| Contact name |
Prof David Bowtell PhD. |
| E-mail(s) |
d.bowtell@petermac.org
|
| Phone |
+613 96561287
|
| Fax |
+613 96561414
|
| URL |
http://www.petermac-research.org.au
|
| Organization name |
PeterMacCallum Cancer Centre
|
| Department |
Research Division
|
| Street address |
Locked Bag 1 A' Beckett St
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
8006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12172 |
Common activation of RAS_MAPK pathway in serous LMP tumours |
|
