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Sample GSM306172 Query DataSets for GSM306172
Status Public on Jul 30, 2008
Title 23036
Sample type RNA
 
Source name Ovarian Tumour, Serous LMP
Organism Homo sapiens
Characteristics LMP
mutant BRAF
Grade High
Stage IIIC
Micropapillary feature Negative
(LMP) implants micro-invasive
Co-exhistant histology NA
LMP Cluster
PRIMARY
arrayed site Ovary
RNA taken from whole tumour sections
Treatment protocol Frozen tissue stored at -80oC taken after surgery
Extracted molecule total RNA
Extraction protocol Trizol extraction and column chromotogrpahy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Affymetrix HG-U133 2.0plus expression array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GS300-7G scanner
Description Gene expression data from human ovarian tumours
Data processing The data were analyzed with Gene Chip Operating Software Version 1.4 using Affymetrix default analysis settings. The CEL files generated was later normalized using the RMA method
 
Submission date Jul 18, 2008
Last update date Jul 30, 2008
Contact name Prof David Bowtell PhD.
E-mail(s) d.bowtell@petermac.org
Phone +613 96561287
Fax +613 96561414
URL http://www.petermac-research.org.au
Organization name PeterMacCallum Cancer Centre
Department Research Division
Street address Locked Bag 1 A' Beckett St
City Melbourne
State/province VIC
ZIP/Postal code 8006
Country Australia
 
Platform ID GPL570
Series (1)
GSE12172 Common activation of RAS_MAPK pathway in serous LMP tumours

Data table header descriptions
ID_REF PROBEID
VALUE RMA normalized signal value

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.73577
AFFX-BioB-M_at 9.13863
AFFX-BioB-3_at 8.59027
AFFX-BioC-5_at 9.73635
AFFX-BioC-3_at 9.90506
AFFX-BioDn-5_at 11.16532
AFFX-BioDn-3_at 12.06734
AFFX-CreX-5_at 13.18265
AFFX-CreX-3_at 13.10106
AFFX-DapX-5_at 7.17002
AFFX-DapX-M_at 9.4999
AFFX-DapX-3_at 10.59966
AFFX-LysX-5_at 4.92983
AFFX-LysX-M_at 5.81238
AFFX-LysX-3_at 8.26435
AFFX-PheX-5_at 5.47869
AFFX-PheX-M_at 6.83099
AFFX-PheX-3_at 7.58319
AFFX-ThrX-5_at 5.29708
AFFX-ThrX-M_at 7.11046

Total number of rows: 46440

Table truncated, full table size 843 Kbytes.




Supplementary file Size Download File type/resource
GSM306172.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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