NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM306177 Query DataSets for GSM306177
Status Public on Jul 30, 2008
Title 23066
Sample type RNA
 
Source name Ovarian Tumour, Serous LMP
Organism Homo sapiens
Characteristics LMP
Wild Type (BRAF/KRAS/ERBB2)
Grade Low
Stage IIIA
Micropapillary feature Negative
(LMP) implants Non-invasive
Co-exhistant histology NA
LMP Cluster
PRIMARY
arrayed site Ovary
RNA taken from whole tumour sections
Treatment protocol Frozen tissue stored at -80oC taken after surgery
Extracted molecule total RNA
Extraction protocol Trizol extraction and column chromotogrpahy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Affymetrix HG-U133 2.0plus expression array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GS300-7G scanner
Description Gene expression data from human ovarian tumours
Data processing The data were analyzed with Gene Chip Operating Software Version 1.4 using Affymetrix default analysis settings. The CEL files generated was later normalized using the RMA method
 
Submission date Jul 18, 2008
Last update date Jul 30, 2008
Contact name Prof David Bowtell PhD.
E-mail(s) d.bowtell@petermac.org
Phone +613 96561287
Fax +613 96561414
URL http://www.petermac-research.org.au
Organization name PeterMacCallum Cancer Centre
Department Research Division
Street address Locked Bag 1 A' Beckett St
City Melbourne
State/province VIC
ZIP/Postal code 8006
Country Australia
 
Platform ID GPL570
Series (1)
GSE12172 Common activation of RAS_MAPK pathway in serous LMP tumours

Data table header descriptions
ID_REF PROBEID
VALUE RMA normalized signal value

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.98677
AFFX-BioB-M_at 8.33645
AFFX-BioB-3_at 7.78404
AFFX-BioC-5_at 9.35234
AFFX-BioC-3_at 9.51089
AFFX-BioDn-5_at 10.67502
AFFX-BioDn-3_at 11.941
AFFX-CreX-5_at 12.79692
AFFX-CreX-3_at 12.77697
AFFX-DapX-5_at 6.72248
AFFX-DapX-M_at 8.58258
AFFX-DapX-3_at 9.43061
AFFX-LysX-5_at 4.06181
AFFX-LysX-M_at 4.89569
AFFX-LysX-3_at 6.79557
AFFX-PheX-5_at 4.76678
AFFX-PheX-M_at 5.46464
AFFX-PheX-3_at 6.51286
AFFX-ThrX-5_at 5.1061
AFFX-ThrX-M_at 6.06832

Total number of rows: 46440

Table truncated, full table size 843 Kbytes.




Supplementary file Size Download File type/resource
GSM306177.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap