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Status |
Public on Oct 15, 2018 |
Title |
Control Sultana_1 [WRKY19 OE] |
Sample type |
RNA |
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|
Source name |
Control plantlet of Vitis vinifera cv. Sultana _line 1
|
Organism |
Vitis vinifera |
Characteristics |
cultivar: Sultana genotype/variation: control tissue: young leaves
|
Treatment protocol |
For transient transformation of Vitis vinifera cv. Sultana, the 35S:VvWRKY19 construct was transferred to Agrobacterium tumefaciens strain C58C1 by electroporation. As control, Agrobacterium was also transformed with an empty GoldenBraid 2.0 vector (pDGBΩ1). Seven in vitro six-week-old plants of grapevine cv. Sultana were immersed in each bacterial suspension and vacuum infiltrated (2 X 2 min at 90kPa).
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Growth protocol |
For the transient overexpression of VvWRKY19 in grapevine, plantlets of Vitis vinifera cv. Sultana were in vitro micropropagated and cultivated in growth chamber at 25°C with a 16-h photoperiod.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from ~100 mg of young and well expanded ground leaves using SpectrumTM Plant Total RNA kit (Sigma-Aldrich) according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
For microarray analysis on Sultana leaves transiently overexpressing VvWRKY19, the cDNA synthesis and labeling reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
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Hybridization protocol |
For microarray analysis on Sultana leaves transiently overexpressing VvWRKY19, the hybridization and washing reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
Scan protocol |
Scanning and Feature Extraction were performed by using an Agilent Scanner following the settings and parameters indicated in the instruction manual.
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Data processing |
A data-matrix was prepared selecting from each single sub-array outcome file the gProcessedSignalvalues, which are the raw fluorescence intensities of each probe. The data were normalized on the 75th percentile and a correlation analysis was then performed to assess the consistency of the biological quadruplicates.
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Submission date |
Apr 16, 2018 |
Last update date |
Oct 15, 2018 |
Contact name |
Silvia Dal Santo |
E-mail(s) |
silvia.dalsanto@univr.it
|
Organization name |
University of Verona
|
Department |
Department of Biotechnology
|
Street address |
Strada Le Grazie, 15
|
City |
Verona |
ZIP/Postal code |
37134 |
Country |
Italy |
|
|
Platform ID |
GPL22427 |
Series (2) |
GSE113223 |
Timing and order of the molecular events marking the onset of berry ripening in grapevine [WRKY19 OE] |
GSE113225 |
Timing and order of the molecular events marking the onset of berry ripening in grapevine |
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