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Sample GSM3100523 Query DataSets for GSM3100523
Status Public on Oct 15, 2018
Title Control Sultana_1 [WRKY19 OE]
Sample type RNA
 
Source name Control plantlet of Vitis vinifera cv. Sultana _line 1
Organism Vitis vinifera
Characteristics cultivar: Sultana
genotype/variation: control
tissue: young leaves
Treatment protocol For transient transformation of Vitis vinifera cv. Sultana, the 35S:VvWRKY19 construct was transferred to Agrobacterium tumefaciens strain C58C1 by electroporation. As control, Agrobacterium was also transformed with an empty GoldenBraid 2.0 vector (pDGBΩ1). Seven in vitro six-week-old plants of grapevine cv. Sultana were immersed in each bacterial suspension and vacuum infiltrated (2 X 2 min at 90kPa).
Growth protocol For the transient overexpression of VvWRKY19 in grapevine, plantlets of Vitis vinifera cv. Sultana were in vitro micropropagated and cultivated in growth chamber at 25°C with a 16-h photoperiod.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from ~100 mg of young and well expanded ground leaves using SpectrumTM Plant Total RNA kit (Sigma-Aldrich) according to the manufacturer's instructions.
Label Cy3
Label protocol For microarray analysis on Sultana leaves transiently overexpressing VvWRKY19, the cDNA synthesis and labeling reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
 
Hybridization protocol For microarray analysis on Sultana leaves transiently overexpressing VvWRKY19, the hybridization and washing reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
Scan protocol Scanning and Feature Extraction were performed by using an Agilent Scanner following the settings and parameters indicated in the instruction manual.
Data processing A data-matrix was prepared selecting from each single sub-array outcome file the gProcessedSignalvalues, which are the raw fluorescence intensities of each probe.
The data were normalized on the 75th percentile and a correlation analysis was then performed to assess the consistency of the biological quadruplicates.
 
Submission date Apr 16, 2018
Last update date Oct 15, 2018
Contact name Silvia Dal Santo
E-mail(s) silvia.dalsanto@univr.it
Organization name University of Verona
Department Department of Biotechnology
Street address Strada Le Grazie, 15
City Verona
ZIP/Postal code 37134
Country Italy
 
Platform ID GPL22427
Series (2)
GSE113223 Timing and order of the molecular events marking the onset of berry ripening in grapevine [WRKY19 OE]
GSE113225 Timing and order of the molecular events marking the onset of berry ripening in grapevine

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
CUST_1_PI428914343 4.29
CUST_10_PI428914343 4.32
CUST_100_PI428914343 850.91
CUST_1000_PI428914343 41.04
CUST_10000_PI428914343 72.12
CUST_10001_PI428914343 7.62
CUST_10002_PI428914343 163.53
CUST_10003_PI428914343 47.77
CUST_10004_PI428914343 89.77
CUST_10005_PI428914343 34.93
CUST_10006_PI428914343 2265.86
CUST_10007_PI428914343 73.12
CUST_10008_PI428914343 1475.94
CUST_10009_PI428914343 2576.79
CUST_1001_PI428914343 4.30
CUST_10010_PI428914343 5.30
CUST_10011_PI428914343 157.86
CUST_10012_PI428914343 3.93
CUST_10013_PI428914343 37.59
CUST_10014_PI428914343 97.56

Total number of rows: 34369

Table truncated, full table size 982 Kbytes.




Supplementary file Size Download File type/resource
GSM3100523_Control_Sultana_1.txt.gz 1.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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