|
| Status |
Public on Apr 25, 2018 |
| Title |
cowpea vs adzuki bean |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Cowpea leaf
|
| Organism |
Vigna unguiculata |
| Characteristics |
cultivar: B128
origin: China
tissue: leaf
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Seedlings were grown in a growth chamber with the ambient temperature cycle of 28°C (day)/22°C (night) and 16h/8h of photoperiod.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extratced using the CTAB method
|
| Label |
Cy3
|
| Label protocol |
Genomic DNA from cowpea and adzuki bean were labeled with fluorochromes Cy3 and Cy5, respectively
|
| |
|
| Channel 2 |
| Source name |
Adzuki bean leaf
|
| Organism |
Vigna angularis |
| Characteristics |
cultivar: Suxiaodou 12
origin: China
tissue: leaf
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Seedlings were grown in a growth chamber with the ambient temperature cycle of 28°C (day)/22°C (night) and 16h/8h of photoperiod.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extratced using the CTAB method
|
| Label |
Cy5
|
| Label protocol |
Genomic DNA from cowpea and adzuki bean were labeled with fluorochromes Cy3 and Cy5, respectively
|
| |
|
| |
| Hybridization protocol |
Genomic DNA from cowpea and adzuki bean were mixed with SeqCap EZ Developer Reagent. The SeqCap EZ Developer Reagent is an alternative of human Cot-1 DNA specifically designed for plants to minimize non-specific binding of probes and DNA. After being denatured at 95°C, the mixture was applied to the microarray, and the hybridization was carried out at 57°C for 40 hours (Oligo aCGH hybridization kit, Agilent Technologies).
|
| Scan protocol |
After hybridization, the microarray was washed with OligoaCGH wash buffer at 37°C, and slides were then dried. The arrays were then scanned using an Agilent Scanner (Agilent Technologies)
|
| Description |
no replicate
|
| Data processing |
The log2-intensities were extracted from raw microarray image files using the Agilent Feature Extraction software version 9.0
|
| |
|
| Submission date |
Apr 25, 2018 |
| Last update date |
Apr 25, 2018 |
| Contact name |
Pei Xu |
| E-mail(s) |
peixu.zaas@gmail.com
|
| Organization name |
Zhejiang Academy of Agricultural Sciences
|
| Department |
Vegetable Institute
|
| Street address |
198 Shiqiao Rd
|
| City |
Hangzhou |
| ZIP/Postal code |
310021 |
| Country |
China |
| |
|
| Platform ID |
GPL24932 |
| Series (1) |
| GSE113644 |
Genes specific to cowpea: cowpea vs adzuki bean |
|
