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Sample GSM31206 Query DataSets for GSM31206
Status Public on May 17, 2005
Title Arrocillo leaves under solar UV-B-3
Sample type RNA
 
Channel 1
Source name leaves under solar UV-B
Organism Zea mays
Extracted molecule total RNA
 
Channel 2
Source name leaves under no UV-B
Organism Zea mays
Extracted molecule total RNA
 
 
Description Field treatments used different plastic filters to screen UV-B (UV-B exclusion) or UV-B lamps (UV-B supplementation). Solar UV-B radiation was removed to produce the minus UV-B treatment using polyester filters (PE, 100 µm clear polyester plastic; Tap Plastics, Mountain View CA). This PE filter absorbs UV-B without significantly affecting UV-A or visible radiation (Casati and Walbot 2003). To control for differences in wind or humidity under plastic sheeting, cellulose acetate sheeting was placed over a control group (CA, 100 µm extra clear cellulose acetate plastic, Tap Plastics); the CA sheeting transmit most radiation from sunlight. Seeds from the six maize lines were planted during summers 2002 and 2003 outdoors at the Stanford Plant Growth Facility. Approximately 14 days after sowing and 7 days after germination, 1.5 m x 3.2 m of each plastic was draped over 1 m x 2.5 m wooden frames; the excess plastic was stapled to the sides of the frames to reduce light exposure. The N and S sides were left open to allow air circulation. However, 50 cm-long curtains with the same plastic were made on the E and W sides to avoid early morning and late afternoon UV exposure. The frames were maintained about 30 cm above the plant canopy during the course of the experiments. Temperature and humidity in the soil and in the leaves were recorded using an infrared thermometer (Model 210ALCS microcomputer based agri-term infrared thermometer, Everest Interscience Inc., Fullerton, CA) and a relative humidity hygrometer (Thermo-Hygro 800016, Sper Scientific, Scottsdale, AZ); there were no differences among the plots. Average canopy temperatures under the filters were always within 0.5 °C of each other, and in no case were consistent differences in temperature detected between filter treatments. The same was observed when humidity was recorded; differences were <25% between treatments. Measurements of incoming solar radiation were obtained using an Optronics model 752 spectroradiometer (Optronics Laboratories, Orlando, FL) that was calibrated against a National Bureau of Standards certified radiation source before each use. The spectrum under each filter was recorded periodically with 1 nm resolution across the entire sunlight spectrum (290 to 800 nm) to check for changes in the transmittance of the filters. Under our conditions, there were no significant reductions in transmittance after 3 weeks of exposure to solar UV-B, equivalent to the duration of our experiments. Consequently the filters were not replaced, but as they accumulate dust and could develop small tears, we cleaned them at least every fourth day and we replaced areas with small rips. Plants were grown under the specified conditions for 21 days, adult leaf samples from adult leaf 9 or 10 were collected for experiments at 4 PM in all treatments. The experiment was repeated using different replicate plots (with at least 10 plants of each genotype per plot) to control the field variables that might influence measurements.
 
Submission date Sep 29, 2004
Last update date Sep 08, 2006
Contact name Paula Casati
E-mail(s) casati@cefobi-conicet.gov.ar
Phone 54-341-4371955
Organization name Universidad Nacional de Rosario
Department Biological Sciences
Lab Casati
Street address Suipacha 531
City Rosario
State/province Santa Fe
ZIP/Postal code 2000
Country Argentina
 
Platform ID GPL498
Series (1)
GSE2163 W23 and High altitude maize under solar and supplementary UV-B

Data table header descriptions
ID_REF
VALUE log 2 ratio of intensities without UV-B/solar UV-B
ratio (M-N)/(J-K)f2 [Cy5/Cy3; PE/CA] ratio of intensities without UV-B/solar UV-B
CH1I Mean Intensity Cy3
CH1B Mean Intensity Cy3 of background
CH1AB Median Intensity Cy3 of background
CH2I Mean Intensity Cy5
CH2B Mean Intensity Cy5 of background
CH2AB Median Intensity Cy5 of background
SPIX See Scanalyze Manual
BGPIX See Scanalyze Manual
EDGE See Scanalyze Manual
RAT2 See Scanalyze Manual
MRAT See Scanalyze Manual
REGR See Scanalyze Manual
CORR See Scanalyze Manual
LFRAT See Scanalyze Manual
CH1GTB1 See Scanalyze Manual
CH2GTB1 See Scanalyze Manual
CH1GTB2 See Scanalyze Manual
CH2GTB2 See Scanalyze Manual
CH1EDGEA See Scanalyze Manual
CH2EDGEA See Scanalyze Manual
FLAG See Scanalyze Manual
CH1KSD See Scanalyze Manual
CH1KSP See Scanalyze Manual
CH2KSD See Scanalyze Manual
CH2KSP See Scanalyze Manual

Data table
ID_REF VALUE ratio (M-N)/(J-K)f2 [Cy5/Cy3; PE/CA] CH1I CH1B CH1AB CH2I CH2B CH2AB SPIX BGPIX EDGE RAT2 MRAT REGR CORR LFRAT CH1GTB1 CH2GTB1 CH1GTB2 CH2GTB2 CH1EDGEA CH2EDGEA FLAG CH1KSD CH1KSP CH2KSD CH2KSP
1 1.019944994 2.027841642 6902 752 795 13744 240 411 156 1193 0 2.196 2.229 2.179 0.9812 2.249 0.9679 1 0.9359 1 0.2439 0.3014 0 0.8585 0.00E+00 0.9249 0.00E+00
2 0.93090143 1.90646683 6055 740 788 11212 240 453 156 985 0 2.064 2.076 2.082 0.9795 2.154 1 0.9872 0.9487 0.9872 0.2769 0.345 0 0.8523 0.00E+00 0.8685 0.00E+00
3 1.035693937 2.050099507 7620 666 724 15669 232 448 156 1010 0 2.22 2.321 2.188 0.9748 2.283 0.9808 1 0.9615 1 0.232 0.2581 0 0.9111 0.00E+00 0.948 0.00E+00
4 1141 544 593 577 216 280 156 1011 0 0.6047 0.6212 0.663 0.8605 0.7394 0.9295 0.8718 0.6923 0.7308 0.1604 0.2314 0 0.626 0.00E+00 0.6287 0.00E+00
5 1097 526 553 646 216 248 156 947 0 0.7531 0.75 0.7676 0.9645 0.7893 0.9615 0.9359 0.6987 0.7756 0.142 0.1805 0 0.741 0.00E+00 0.6929 0.00E+00
6 1068 562 570 712 228 256 156 976 0 0.9565 0.8714 0.8944 0.9569 0.9319 0.859 0.8974 0.6474 0.7564 0.1208 0.1842 0 0.6486 0.00E+00 0.6627 0.00E+00
7 1422 582 620 1617 232 272 156 1009 0 1.649 1.606 1.588 0.9738 1.651 0.9679 0.9936 0.7628 0.9359 0.1536 0.2632 0 0.7333 0.00E+00 0.811 0.00E+00
8 1370 580 649 1551 214 258 156 1009 0 1.692 1.772 1.574 0.9477 1.704 0.9038 0.9103 0.6667 0.8397 0.125 0.256 0 0.5755 3.64E-40 0.6968 0.00E+00
9 1470 568 619 1621 204 235 156 997 0 1.571 1.624 1.474 0.9511 1.583 0.8718 0.9103 0.7179 0.8718 0.1472 0.2753 0 0.6791 0.00E+00 0.743 0.00E+00
10 0.363813169 1.286822591 2661 574 643 3122 214 261 156 985 0 1.393 1.43 1.278 0.9612 1.345 1 1 0.9679 1 0.173 0.2091 0 0.8919 0.00E+00 0.972 0.00E+00
11 0.288866115 1.221679722 2625 590 717 2910 218 374 156 990 0 1.323 1.227 1.244 0.961 1.308 1 0.9615 0.9551 0.9615 0.1757 0.2377 0 0.8165 0.00E+00 0.7684 0.00E+00
12 0.340131282 1.26587178 2719 596 728 3124 214 518 156 988 0 1.371 1.322 1.232 0.9247 1.362 0.9872 1 0.9231 1 0.1938 0.227 0 0.7817 0.00E+00 0.8397 0.00E+00
13 0.528276651 1.442205402 24459 602 771 37460 204 749 156 1023 0 1.562 1.566 1.389 0.9595 1.469 1 1 1 1 0.2861 0.248 0 0.9555 0.00E+00 0.9652 0.00E+00
14 0.519915634 1.433871396 25913 568 901 39553 202 1090 156 1016 0 1.553 1.537 1.394 0.9712 1.45 1 1 1 1 0.2702 0.2366 0 0.9384 0.00E+00 0.9572 0.00E+00
15 0.543812899 1.457820304 26781 548 898 41612 202 1087 156 1002 0 1.579 1.599 1.446 0.9744 1.499 1 1 1 1 0.2498 0.2194 0 0.9658 0.00E+00 0.9631 0.00E+00
16 -0.125654723 0.916587983 3731 534 723 3373 200 632 156 1012 0 0.9925 1.094 0.9016 0.8635 1.051 0.9295 0.9103 0.8782 0.8974 0.2693 0.3218 0 0.7379 0.00E+00 0.7276 0.00E+00
17 0.15478919 1.113258934 3372 510 554 3646 196 258 156 1000 0 1.205 1.156 1.197 0.9542 1.268 1 1 1 1 0.2073 0.2694 0 0.9295 0.00E+00 0.9128 0.00E+00
18 0.146223663 1.106668908 3600 514 559 3896 198 282 156 956 0 1.198 1.157 1.158 0.9481 1.235 1 1 0.9615 1 0.2314 0.2746 0 0.9205 0.00E+00 0.8753 0.00E+00
19 0.752591836 1.684816927 3804 542 612 6153 202 350 156 932 0 1.824 1.72 1.713 0.9624 1.816 1 1 1 1 0.1843 0.2198 0 0.9336 0.00E+00 0.9164 0.00E+00
20 0.706899053 1.632291875 3437 560 697 5283 198 446 156 960 0 1.767 1.738 1.646 0.9704 1.721 1 1 0.9936 1 0.1816 0.209 0 0.867 0.00E+00 0.9207 0.00E+00

Total number of rows: 16928

Table truncated, full table size 2625 Kbytes.




Supplementary data files not provided

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