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Status
Public on Jan 01, 2009
Title
HDAC8 KD, No R1881, rep2
Sample type
RNA
Source name
LNCaP Cells
Organism
Homo sapiens
Characteristics
n/a
Treatment protocol
As per title for 16 hours, all in the presence of cyclohexamide
Growth protocol
LNCaP cells were grown in charcoal stripped medium
Extracted molecule
total RNA
Extraction protocol
RNAeasy Kit
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix protocol
Scan protocol
standard Affymetrix protocol
Description
none
Data processing
Data was analyzed using GeneSpring. Gene with intensity >250 either unstimulated or stimulated with R1881 was included for analysis. Data was normaled by medium of the chip. No per gene normalization was performed.
Submission date
Aug 13, 2008
Last update date
Aug 14, 2008
Contact name
Yu Chen
E-mail(s)
cheny1@mskcc.org
Phone
646-888-3356
Organization name
Memorial Sloan Kettering Cancer Center
Department
Human Oncology and Pathogenesis Program
Lab
Chen
Street address
1275 York Ave, Box 20
City
New York
State/province
NY
ZIP/Postal code
10065
Country
USA
Platform ID
GPL571
Series (1)
GSE12438
Effect of individual HDAC knockdown on expression of androgen induced genes
Data table header descriptions
ID_REF
VALUE
Signal
ABS_CALL
indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
223.626
P
0.000753643
AFFX-BioB-M_at
301.473
P
9.4506e-05
AFFX-BioB-3_at
203.081
P
0.000146581
AFFX-BioC-5_at
527.809
P
0.000195116
AFFX-BioC-3_at
732.474
P
5.16732e-05
AFFX-BioDn-5_at
1245.74
P
5.16732e-05
AFFX-BioDn-3_at
3400.8
P
0.000340305
AFFX-CreX-5_at
8243.4
P
5.16732e-05
AFFX-CreX-3_at
9285.54
P
4.42873e-05
AFFX-DapX-5_at
7.98078
A
0.574038
AFFX-DapX-M_at
7.56736
A
0.340661
AFFX-DapX-3_at
3.94246
A
0.724854
AFFX-LysX-5_at
9.34147
A
0.300606
AFFX-LysX-M_at
3.46332
A
0.83416
AFFX-LysX-3_at
7.87285
A
0.275146
AFFX-PheX-5_at
1.19182
A
0.979978
AFFX-PheX-M_at
1.28623
A
0.904333
AFFX-PheX-3_at
5.19248
A
0.876428
AFFX-ThrX-5_at
21.0056
A
0.440646
AFFX-ThrX-M_at
9.16914
A
0.425962
Total number of rows: 22277 Table truncated, full table size 674 Kbytes .Supplementary file
Size
Download
File type/resource
GSM312340.CEL.gz
3.5 Mb
(ftp) (http)
CEL
GSM312340.CHP.gz
6.0 Mb
(ftp) (http)
CHP
GSM312340.EXP.gz
356 b
(ftp) (http)
EXP
Processed data included within Sample table
Processed data provided as supplementary file