|
| Status |
Public on Jan 01, 2009 |
| Title |
HDAC8 KD, 1nM R1881, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP Cells
|
| Organism |
Homo sapiens |
| Characteristics |
n/a
|
| Treatment protocol |
As per title for 16 hours, all in the presence of cyclohexamide
|
| Growth protocol |
LNCaP cells were grown in charcoal stripped medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy Kit
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
none
|
| Data processing |
Data was analyzed using GeneSpring. Gene with intensity >250 either unstimulated or stimulated with R1881 was included for analysis. Data was normaled by medium of the chip. No per gene normalization was performed.
|
| |
|
| Submission date |
Aug 13, 2008 |
| Last update date |
Aug 14, 2008 |
| Contact name |
Yu Chen |
| E-mail(s) |
cheny1@mskcc.org
|
| Phone |
646-888-3356
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Human Oncology and Pathogenesis Program
|
| Lab |
Chen
|
| Street address |
1275 York Ave, Box 20
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE12438 |
Effect of individual HDAC knockdown on expression of androgen induced genes |
|
