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Sample GSM31244 Query DataSets for GSM31244
Status Public on May 17, 2005
Title W23 b,pl leaves under supplementary UV-B-3
Sample type RNA
 
Channel 1
Source name leaves under supplementary UV-B
Organism Zea mays
Extracted molecule total RNA
 
Channel 2
Source name leaves under solar UV-B
Organism Zea mays
Extracted molecule total RNA
 
 
Description Field treatments used different plastic filters to screen UV-B (UV-B exclusion) or UV-B lamps (UV-B supplementation). Solar UV-B radiation was removed to produce the minus UV-B treatment using polyester filters (PE, 100 µm clear polyester plastic; Tap Plastics, Mountain View CA). This PE filter absorbs UV-B without significantly affecting UV-A or visible radiation (Casati and Walbot 2003). To control for differences in wind or humidity under plastic sheeting, cellulose acetate sheeting was placed over a control group (CA, 100 µm extra clear cellulose acetate plastic, Tap Plastics); the CA sheeting transmit most radiation from sunlight. Seeds from the six maize lines were planted during summers 2002 and 2003 outdoors at the Stanford Plant Growth Facility. Approximately 14 days after sowing and 7 days after germination, 1.5 m x 3.2 m of each plastic was draped over 1 m x 2.5 m wooden frames; the excess plastic was stapled to the sides of the frames to reduce light exposure. The N and S sides were left open to allow air circulation. However, 50 cm-long curtains with the same plastic were made on the E and W sides to avoid early morning and late afternoon UV exposure. The frames were maintained about 30 cm above the plant canopy during the course of the experiments. Temperature and humidity in the soil and in the leaves were recorded using an infrared thermometer (Model 210ALCS microcomputer based agri-term infrared thermometer, Everest Interscience Inc., Fullerton, CA) and a relative humidity hygrometer (Thermo-Hygro 800016, Sper Scientific, Scottsdale, AZ); there were no differences among the plots. Average canopy temperatures under the filters were always within 0.5 °C of each other, and in no case were consistent differences in temperature detected between filter treatments. The same was observed when humidity was recorded; differences were <25% between treatments. Measurements of incoming solar radiation were obtained using an Optronics model 752 spectroradiometer (Optronics Laboratories, Orlando, FL) that was calibrated against a National Bureau of Standards certified radiation source before each use. The spectrum under each filter was recorded periodically with 1 nm resolution across the entire sunlight spectrum (290 to 800 nm) to check for changes in the transmittance of the filters. Under our conditions, there were no significant reductions in transmittance after 3 weeks of exposure to solar UV-B, equivalent to the duration of our experiments. Consequently the filters were not replaced, but as they accumulate dust and could develop small tears, we cleaned them at least every fourth day and we replaced areas with small rips. Plants were grown under the specified conditions for 21 days, adult leaf samples from adult leaf 9 or 10 were collected for experiments at 4 PM in all treatments. The experiment was repeated using different replicate plots (with at least 10 plants of each genotype per plot) to control the field variables that might influence measurements.
 
Submission date Sep 29, 2004
Last update date Sep 08, 2006
Contact name Paula Casati
E-mail(s) casati@cefobi-conicet.gov.ar
Phone 54-341-4371955
Organization name Universidad Nacional de Rosario
Department Biological Sciences
Lab Casati
Street address Suipacha 531
City Rosario
State/province Santa Fe
ZIP/Postal code 2000
Country Argentina
 
Platform ID GPL498
Series (1)
GSE2163 W23 and High altitude maize under solar and supplementary UV-B

Data table header descriptions
ID_REF
VALUE log 2 ratio supplemental UV-B/solar UV-B
ratio (J-K)/(M-N)f2 [Cy3/Cy5;UV-B/SL] ratio supplemental UV-B/solar UV-B
CH1I Mean Intensity Cy3
CH1B Mean Intensity Cy3 of background
CH1AB Median Intensity Cy3 of background
CH2I Mean Intensity Cy5
CH2B Mean Intensity Cy5 of background
CH2AB Median Intensity Cy5 of background
SPIX See Scanalyze Manual
BGPIX See Scanalyze Manual
EDGE See Scanalyze Manual
RAT2 See Scanalyze Manual
MRAT See Scanalyze Manual
REGR See Scanalyze Manual
CORR See Scanalyze Manual
LFRAT See Scanalyze Manual
CH1GTB1 See Scanalyze Manual
CH2GTB1 See Scanalyze Manual
CH1GTB2 See Scanalyze Manual
CH2GTB2 See Scanalyze Manual
CH1EDGEA See Scanalyze Manual
CH2EDGEA See Scanalyze Manual
FLAG See Scanalyze Manual
CH1KSD See Scanalyze Manual
CH1KSP See Scanalyze Manual
CH2KSD See Scanalyze Manual
CH2KSP See Scanalyze Manual

Data table
ID_REF VALUE ratio (J-K)/(M-N)f2 [Cy3/Cy5;UV-B/SL] CH1I CH1B CH1AB CH2I CH2B CH2AB SPIX BGPIX EDGE RAT2 MRAT REGR CORR LFRAT CH1GTB1 CH2GTB1 CH1GTB2 CH2GTB2 CH1EDGEA CH2EDGEA FLAG CH1KSD CH1KSP CH2KSD CH2KSP
1 -0.388300532 0.764029087 10466 315 376 8798 619 669 156 1219 0 1.241 1.221 1.202 0.9828 1.228 0.9038 0.9103 0.7756 0.8397 0.3222 0.3372 0 0.7655 0.00E+00 0.7823 0.00E+00
2 -0.386520521 0.764972335 9530 315 395 8058 624 688 156 1032 0 1.24 1.225 1.201 0.9839 1.224 0.891 0.8718 0.7244 0.7628 0.3122 0.3308 0 0.7046 0.00E+00 0.7168 0.00E+00
3 -0.441019153 0.736614064 10653 307 319 8657 620 626 156 1018 0 1.287 1.276 1.249 0.9848 1.273 0.9167 0.9679 0.7756 0.8462 0.3397 0.3614 0 0.7817 0.00E+00 0.776 0.00E+00
4 866 303 308 1396 625 628 156 1009 0 0.7302 0.7301 0.7261 0.9418 0.759 0.8526 0.8205 0.5321 0.5769 0.1647 0.2076 0 0.596 4.75E-43 0.5509 7.70E-37
5 860 302 309 1419 622 628 156 1009 0 0.7001 0.677 0.6745 0.9308 0.7081 0.7692 0.7692 0.5641 0.5962 0.1505 0.2076 0 0.5591 6.14E-38 0.5521 5.27E-37
6 934 300 309 1438 605 633 156 1009 0 0.7611 0.7892 0.4746 0.7768 0.5381 0.8526 0.8077 0.6282 0.6474 0.1529 0.2097 0 0.6307 0.00E+00 0.6106 4.20E-45
7 0.666795767 1.587543107 4635 298 308 7872 611 634 156 1009 0 0.5973 0.6032 0.5804 0.971 0.5894 0.859 0.8462 0.5833 0.641 0.3584 0.3736 0 0.6005 1.08E-43 0.6014 7.85E-44
8 0.713162118 1.639393426 4933 293 306 8633 611 652 156 1009 0 0.5784 0.5967 0.5736 0.973 0.5817 0.8846 0.8205 0.5897 0.6859 0.3681 0.3961 0 0.5902 3.20E-42 0.6269 0.00E+00
9 0.722137295 1.649624077 4331 290 300 7627 597 615 156 1009 0 0.5748 0.5999 0.557 0.976 0.5636 0.8013 0.859 0.6282 0.6731 0.3733 0.3719 0 0.6262 0.00E+00 0.6252 0.00E+00
10 0.222702423 1.166917383 2265 292 296 3020 592 597 156 1009 0 0.8126 0.8119 0.7721 0.9643 0.7943 0.859 0.8077 0.6282 0.6474 0.2748 0.3011 0 0.639 0.00E+00 0.6331 0.00E+00
11 0.28179143 1.215703516 2603 295 302 3565 606 611 156 1009 0 0.78 0.7186 0.7841 0.9607 0.8095 0.8462 0.8782 0.641 0.6731 0.3164 0.3557 0 0.6429 0.00E+00 0.6289 0.00E+00
12 0.035613897 1.024992886 3013 295 304 3555 617 626 156 1009 0 0.9251 0.9445 0.8863 0.973 0.9085 0.8205 0.8846 0.7179 0.7564 0.2596 0.2964 0 0.7105 0.00E+00 0.71 0.00E+00
13 -0.151073424 0.900580145 32362 290 307 31066 606 624 156 1009 0 1.053 1.038 1.031 0.9943 1.037 0.8974 0.9231 0.7179 0.8141 0.3954 0.4005 0 0.7073 0.00E+00 0.7641 0.00E+00
14 -0.155223223 0.897993419 35102 284 304 33557 584 600 156 1009 0 1.056 1.056 1.032 0.9924 1.041 0.9295 0.9936 0.8077 0.9359 0.3862 0.3817 0 0.8268 0.00E+00 0.8696 0.00E+00
15 -0.151328454 0.900420961 37855 278 287 36247 565 571 156 1009 0 1.053 1.038 1.035 0.9939 1.042 0.9744 0.9744 0.8333 0.9423 0.3798 0.3781 0 0.8589 0.00E+00 0.887 0.00E+00
16 -0.203758379 0.868285635 4931 282 288 4821 564 571 156 987 0 1.092 1.102 1.075 0.9878 1.089 0.8526 0.8718 0.6474 0.6987 0.3172 0.3692 0 0.6552 0.00E+00 0.6788 0.00E+00
17 -0.191214125 0.875868309 4894 282 287 4829 569 577 156 1012 0 1.083 1.097 1.078 0.9896 1.09 0.9038 0.9038 0.6923 0.7885 0.3002 0.3425 0 0.6997 0.00E+00 0.7499 0.00E+00
18 -0.18389308 0.880324253 4677 279 286 4649 566 573 156 1037 0 1.077 1.093 1.061 0.9878 1.075 0.8846 0.8654 0.6795 0.7308 0.3313 0.3818 0 0.6925 0.00E+00 0.7044 0.00E+00
19 -0.106459608 0.928864713 5122 277 297 5317 571 586 156 1009 0 1.021 1.04 1.006 0.9804 1.027 0.9103 0.9167 0.8077 0.8205 0.2798 0.3054 0 0.8062 0.00E+00 0.8027 0.00E+00
20 -0.057268369 0.961082132 4931 279 374 5294 579 653 156 1009 0 0.9866 0.9892 0.9272 0.9795 0.9455 0.9359 0.9359 0.8654 0.8974 0.303 0.3192 0 0.8381 0.00E+00 0.845 0.00E+00

Total number of rows: 16928

Table truncated, full table size 2850 Kbytes.




Supplementary data files not provided

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