NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM31247 Query DataSets for GSM31247
Status Public on May 17, 2005
Title Arrocillo leaves under supplemental UV-B-3
Sample type RNA
 
Channel 1
Source name leaves under supplemental UV-B
Organism Zea mays
Extracted molecule total RNA
 
Channel 2
Source name leaves under solar UV-B
Organism Zea mays
Extracted molecule total RNA
 
 
Description Field treatments used different plastic filters to screen UV-B (UV-B exclusion) or UV-B lamps (UV-B supplementation). Solar UV-B radiation was removed to produce the minus UV-B treatment using polyester filters (PE, 100 µm clear polyester plastic; Tap Plastics, Mountain View CA). This PE filter absorbs UV-B without significantly affecting UV-A or visible radiation (Casati and Walbot 2003). To control for differences in wind or humidity under plastic sheeting, cellulose acetate sheeting was placed over a control group (CA, 100 µm extra clear cellulose acetate plastic, Tap Plastics); the CA sheeting transmit most radiation from sunlight. Seeds from the six maize lines were planted during summers 2002 and 2003 outdoors at the Stanford Plant Growth Facility. Approximately 14 days after sowing and 7 days after germination, 1.5 m x 3.2 m of each plastic was draped over 1 m x 2.5 m wooden frames; the excess plastic was stapled to the sides of the frames to reduce light exposure. The N and S sides were left open to allow air circulation. However, 50 cm-long curtains with the same plastic were made on the E and W sides to avoid early morning and late afternoon UV exposure. The frames were maintained about 30 cm above the plant canopy during the course of the experiments. Temperature and humidity in the soil and in the leaves were recorded using an infrared thermometer (Model 210ALCS microcomputer based agri-term infrared thermometer, Everest Interscience Inc., Fullerton, CA) and a relative humidity hygrometer (Thermo-Hygro 800016, Sper Scientific, Scottsdale, AZ); there were no differences among the plots. Average canopy temperatures under the filters were always within 0.5 °C of each other, and in no case were consistent differences in temperature detected between filter treatments. The same was observed when humidity was recorded; differences were <25% between treatments. Measurements of incoming solar radiation were obtained using an Optronics model 752 spectroradiometer (Optronics Laboratories, Orlando, FL) that was calibrated against a National Bureau of Standards certified radiation source before each use. The spectrum under each filter was recorded periodically with 1 nm resolution across the entire sunlight spectrum (290 to 800 nm) to check for changes in the transmittance of the filters. Under our conditions, there were no significant reductions in transmittance after 3 weeks of exposure to solar UV-B, equivalent to the duration of our experiments. Consequently the filters were not replaced, but as they accumulate dust and could develop small tears, we cleaned them at least every fourth day and we replaced areas with small rips. Plants were grown under the specified conditions for 21 days, adult leaf samples from adult leaf 9 or 10 were collected for experiments at 4 PM in all treatments. The experiment was repeated using different replicate plots (with at least 10 plants of each genotype per plot) to control the field variables that might influence measurements.
 
Submission date Sep 29, 2004
Last update date Sep 08, 2006
Contact name Paula Casati
E-mail(s) casati@cefobi-conicet.gov.ar
Phone 54-341-4371955
Organization name Universidad Nacional de Rosario
Department Biological Sciences
Lab Casati
Street address Suipacha 531
City Rosario
State/province Santa Fe
ZIP/Postal code 2000
Country Argentina
 
Platform ID GPL498
Series (1)
GSE2163 W23 and High altitude maize under solar and supplementary UV-B

Data table header descriptions
ID_REF
VALUE log 2 ratio supplemental UV-B/solar UV-B
ratio (J-K)/(M-N)f2 [Cy3/Cy5;UV-B/SL] ratio supplemental UV-B/solar UV-B
CH1I Mean Intensity Cy3
CH1B Mean Intensity Cy3 of background
CH1AB Median Intensity Cy3 of background
CH2I Mean Intensity Cy5
CH2B Mean Intensity Cy5 of background
CH2AB Median Intensity Cy5 of background
SPIX See Scanalyze Manual
BGPIX See Scanalyze Manual
EDGE See Scanalyze Manual
RAT2 See Scanalyze Manual
MRAT See Scanalyze Manual
REGR See Scanalyze Manual
CORR See Scanalyze Manual
LFRAT See Scanalyze Manual
CH1GTB1 See Scanalyze Manual
CH2GTB1 See Scanalyze Manual
CH1GTB2 See Scanalyze Manual
CH2GTB2 See Scanalyze Manual
CH1EDGEA See Scanalyze Manual
CH2EDGEA See Scanalyze Manual
FLAG See Scanalyze Manual
CH1KSD See Scanalyze Manual
CH1KSP See Scanalyze Manual
CH2KSD See Scanalyze Manual
CH2KSP See Scanalyze Manual

Data table
ID_REF VALUE ratio (J-K)/(M-N)f2 [Cy3/Cy5;UV-B/SL] CH1I CH1B CH1AB CH2I CH2B CH2AB SPIX BGPIX EDGE RAT2 MRAT REGR CORR LFRAT CH1GTB1 CH2GTB1 CH1GTB2 CH2GTB2 CH1EDGEA CH2EDGEA FLAG CH1KSD CH1KSP CH2KSD CH2KSP
1 -0.039445735 0.974847644 34368 422 894 33109 291 709 156 1213 0 0.9668 0.9622 0.9341 0.9752 0.9568 0.9551 0.9808 0.8526 0.8846 0.3259 0.3049 0 0.8082 0.00E+00 0.8221 0.00E+00
2 -0.207710087 0.867529255 20595 425 714 22202 290 506 156 1004 0 1.086 1.08 1.049 0.9736 1.079 0.9744 0.9487 0.8397 0.8718 0.305 0.3005 0 0.8035 0.00E+00 0.8316 0.00E+00
3 -0.101676699 0.933691402 29179 410 483 29316 277 304 156 1019 0 1.009 0.9989 0.9455 0.9539 0.9908 0.9487 0.9808 0.8846 0.9551 0.3307 0.3175 0 0.8694 0.00E+00 0.9187 0.00E+00
4 0.234203369 1.178455899 1904 386 400 1478 264 277 156 1023 0 0.7997 0.75 0.7693 0.9549 0.7975 0.7885 0.8269 0.5705 0.5641 0.2745 0.3039 0 0.5471 2.13E-36 0.5184 1.01E-32
5 0.145988849 1.108557228 1597 369 380 1311 267 277 156 1018 0 0.8502 0.8126 0.8131 0.9459 0.8522 0.8013 0.8718 0.5321 0.5962 0.2612 0.2875 0 0.5495 1.08E-36 0.5362 5.75E-35
6 0.235201987 1.179271896 1824 355 374 1440 266 277 156 1009 0 0.7992 0.8246 0.7772 0.9434 0.8145 0.8205 0.8333 0.5705 0.6026 0.2915 0.3074 0 0.5713 1.38E-39 0.5408 1.60E-35
7 0.742022748 1.675645714 4692 352 371 2709 268 282 156 1007 0 0.5624 0.6207 0.4839 0.905 0.5049 0.8397 0.859 0.6731 0.6859 0.3617 0.3476 0 0.6522 0.00E+00 0.6226 0.00E+00
8 0.64911572 1.571138237 4479 358 408 2745 273 317 156 1009 0 0.5999 0.6509 0.5617 0.9234 0.5857 0.8974 0.8077 0.6538 0.6603 0.37 0.3683 0 0.6256 0.00E+00 0.6137 1.40E-45
9 0.671060634 1.595219594 4907 364 442 2958 274 330 156 1033 0 0.5908 0.6289 0.5509 0.9367 0.5692 0.859 0.8269 0.6795 0.6667 0.3615 0.3301 0 0.6388 0.00E+00 0.5992 1.25E-43
10 -0.767389661 0.588577681 5048 371 436 7765 276 315 156 1023 0 1.601 1.632 1.459 0.9402 1.593 0.9295 0.9679 0.8526 0.9295 0.2751 0.3075 0 0.8227 0.00E+00 0.8844 0.00E+00
11 -0.744948256 0.5978047 5108 371 402 7750 282 320 156 1009 0 1.577 1.593 1.487 0.9404 1.625 0.9808 0.9744 0.9167 0.9615 0.2697 0.2793 0 0.9018 0.00E+00 0.9122 0.00E+00
12 -0.706035456 0.614148288 5057 371 875 7469 278 782 156 1009 0 1.535 1.542 1.121 0.8897 1.295 0.9487 0.9487 0.8782 0.8846 0.2873 0.2824 0 0.8405 0.00E+00 0.8193 0.00E+00
13 -0.115863829 0.924554694 47943 371 1081 48773 280 981 156 1009 0 1.019 1.001 0.971 0.9637 1.008 0.9359 0.9615 0.859 0.9103 0.2761 0.2758 0 0.8104 0.00E+00 0.8588 0.00E+00
14 -0.147145957 0.904723245 50050 367 825 52032 277 842 156 1009 0 1.042 1.001 0.9797 0.9732 1.007 0.9936 0.9936 0.9423 0.9872 0.28 0.2541 0 0.8967 0.00E+00 0.9278 0.00E+00
15 -0.094732692 0.938196295 47296 363 625 47418 272 629 156 1009 0 1.005 1.001 0.9876 0.988 0.9996 1 0.9872 0.9423 0.9487 0.2907 0.2844 0 0.9031 0.00E+00 0.8938 0.00E+00
16 -0.068469893 0.955431606 4253 351 378 4114 265 281 156 997 0 0.9864 1.011 0.9272 0.9685 0.956 0.8846 0.8718 0.6859 0.7244 0.3504 0.3662 0 0.67 0.00E+00 0.6668 0.00E+00
17 0.029024437 1.022229293 4959 346 382 4519 266 294 156 995 0 0.922 0.9897 0.8351 0.9463 0.8762 0.9038 0.9038 0.7179 0.7308 0.384 0.3939 0 0.6878 0.00E+00 0.681 0.00E+00
18 -0.030871757 0.980658441 4308 355 490 4067 268 379 156 948 0 0.961 0.9947 0.9042 0.9569 0.9424 0.859 0.8654 0.6859 0.7244 0.3051 0.307 0 0.6452 0.00E+00 0.6302 0.00E+00
19 -0.387974563 0.765630304 7520 367 574 9071 266 472 156 1023 0 1.231 1.237 1.146 0.9704 1.187 0.9615 0.9936 0.9103 0.9615 0.2693 0.271 0 0.8589 0.00E+00 0.887 0.00E+00
20 -0.270732379 0.830448163 3793 375 591 4147 268 514 156 980 0 1.135 1.096 1.036 0.908 1.156 0.9679 0.9167 0.7821 0.7564 0.3553 0.3861 0 0.7259 0.00E+00 0.6449 0.00E+00

Total number of rows: 16928

Table truncated, full table size 2861 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap