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Sample GSM31248 Query DataSets for GSM31248
Status Public on May 17, 2005
Title Cacahuacintle leaves under supplemental UV-B-3
Sample type RNA
 
Channel 1
Source name leaves under supplemental UV-B
Organism Zea mays
Extracted molecule total RNA
 
Channel 2
Source name leaves under solar UV-B
Organism Zea mays
Extracted molecule total RNA
 
 
Description Field treatments used different plastic filters to screen UV-B (UV-B exclusion) or UV-B lamps (UV-B supplementation). Solar UV-B radiation was removed to produce the minus UV-B treatment using polyester filters (PE, 100 µm clear polyester plastic; Tap Plastics, Mountain View CA). This PE filter absorbs UV-B without significantly affecting UV-A or visible radiation (Casati and Walbot 2003). To control for differences in wind or humidity under plastic sheeting, cellulose acetate sheeting was placed over a control group (CA, 100 µm extra clear cellulose acetate plastic, Tap Plastics); the CA sheeting transmit most radiation from sunlight. Seeds from the six maize lines were planted during summers 2002 and 2003 outdoors at the Stanford Plant Growth Facility. Approximately 14 days after sowing and 7 days after germination, 1.5 m x 3.2 m of each plastic was draped over 1 m x 2.5 m wooden frames; the excess plastic was stapled to the sides of the frames to reduce light exposure. The N and S sides were left open to allow air circulation. However, 50 cm-long curtains with the same plastic were made on the E and W sides to avoid early morning and late afternoon UV exposure. The frames were maintained about 30 cm above the plant canopy during the course of the experiments. Temperature and humidity in the soil and in the leaves were recorded using an infrared thermometer (Model 210ALCS microcomputer based agri-term infrared thermometer, Everest Interscience Inc., Fullerton, CA) and a relative humidity hygrometer (Thermo-Hygro 800016, Sper Scientific, Scottsdale, AZ); there were no differences among the plots. Average canopy temperatures under the filters were always within 0.5 °C of each other, and in no case were consistent differences in temperature detected between filter treatments. The same was observed when humidity was recorded; differences were <25% between treatments. Measurements of incoming solar radiation were obtained using an Optronics model 752 spectroradiometer (Optronics Laboratories, Orlando, FL) that was calibrated against a National Bureau of Standards certified radiation source before each use. The spectrum under each filter was recorded periodically with 1 nm resolution across the entire sunlight spectrum (290 to 800 nm) to check for changes in the transmittance of the filters. Under our conditions, there were no significant reductions in transmittance after 3 weeks of exposure to solar UV-B, equivalent to the duration of our experiments. Consequently the filters were not replaced, but as they accumulate dust and could develop small tears, we cleaned them at least every fourth day and we replaced areas with small rips. Plants were grown under the specified conditions for 21 days, adult leaf samples from adult leaf 9 or 10 were collected for experiments at 4 PM in all treatments. The experiment was repeated using different replicate plots (with at least 10 plants of each genotype per plot) to control the field variables that might influence measurements.
 
Submission date Sep 29, 2004
Last update date Sep 08, 2006
Contact name Paula Casati
E-mail(s) casati@cefobi-conicet.gov.ar
Phone 54-341-4371955
Organization name Universidad Nacional de Rosario
Department Biological Sciences
Lab Casati
Street address Suipacha 531
City Rosario
State/province Santa Fe
ZIP/Postal code 2000
Country Argentina
 
Platform ID GPL498
Series (1)
GSE2163 W23 and High altitude maize under solar and supplementary UV-B

Data table header descriptions
ID_REF
VALUE log 2 ratio intensity supplemental UV-B/solar UV-B
ratio (J-K)/(M-N)f2 [Cy3/Cy5;UV-B/SL] ratio intensity supplemental UV-B/solar UV-B
CH1I Mean Intensity Cy3
CH1B Mean Intensity Cy3 of background
CH1AB Median Intensity Cy3 of background
CH2I Mean Intensity Cy5
CH2B Mean Intensity Cy5 of background
CH2AB Median Intensity Cy5 of background
SPIX See Scanalyze Manual
BGPIX See Scanalyze Manual
EDGE See Scanalyze Manual
RAT2 See Scanalyze Manual
MRAT See Scanalyze Manual
REGR See Scanalyze Manual
CORR See Scanalyze Manual
LFRAT See Scanalyze Manual
CH1GTB1 See Scanalyze Manual
CH2GTB1 See Scanalyze Manual
CH1GTB2 See Scanalyze Manual
CH2GTB2 See Scanalyze Manual
CH1EDGEA See Scanalyze Manual
CH2EDGEA See Scanalyze Manual
FLAG See Scanalyze Manual
CH1KSD See Scanalyze Manual
CH1KSP See Scanalyze Manual
CH2KSD See Scanalyze Manual
CH2KSP See Scanalyze Manual

Data table
ID_REF VALUE ratio (J-K)/(M-N)f2 [Cy3/Cy5;UV-B/SL] CH1I CH1B CH1AB CH2I CH2B CH2AB SPIX BGPIX EDGE RAT2 MRAT REGR CORR LFRAT CH1GTB1 CH2GTB1 CH1GTB2 CH2GTB2 CH1EDGEA CH2EDGEA FLAG CH1KSD CH1KSP CH2KSD CH2KSP
1 -0.506597258 0.70389372 30957 474 1672 43476 432 2096 156 1178 0 1.412 1.441 1.389 0.9854 1.417 1 1 0.9615 0.9808 0.2194 0.2252 0 0.8982 0.00E+00 0.9055 0.00E+00
2 -0.534264146 0.690523602 24455 459 535 34958 418 558 156 970 0 1.439 1.518 1.391 0.9793 1.43 0.9679 0.9551 0.8526 0.9167 0.412 0.4053 0 0.8432 0.00E+00 0.8634 0.00E+00
3 -0.497302911 0.708443089 25939 455 471 36159 405 486 156 1031 0 1.403 1.48 1.31 0.9761 1.351 0.9744 0.9615 0.8846 0.9038 0.4007 0.3828 0 0.8739 0.00E+00 0.8613 0.00E+00
4 -0.146213598 0.903635704 2083 442 472 2158 353 418 156 1040 0 1.1 1.11 1.082 0.9679 1.122 0.7949 0.7949 0.6346 0.6346 0.2599 0.2956 0 0.6112 1.40E-45 0.5753 2.69E-40
5 -0.189160414 0.877132297 2113 431 491 2242 336 404 156 1009 0 1.133 1.146 1.093 0.9826 1.115 0.8333 0.8846 0.6474 0.6923 0.2162 0.2431 0 0.6122 2.80E-45 0.6028 5.05E-44
6 -0.115592618 0.923020221 3190 444 508 3308 351 408 156 1029 0 1.077 1.151 0.9026 0.9052 0.9969 0.8333 0.7949 0.5833 0.6218 0.3174 0.3525 0 0.5528 3.50E-37 0.5512 5.59E-37
7 -0.063399371 0.957024263 14019 462 533 14429 349 417 156 970 0 1.039 1.032 1.035 0.981 1.056 0.9038 0.9359 0.7821 0.8141 0.3701 0.4026 0 0.7295 0.00E+00 0.758 0.00E+00
8 0.010566997 1.007370071 12181 476 604 11898 349 480 156 984 0 0.9867 0.9554 0.9845 0.9867 0.9978 0.8846 0.8846 0.7692 0.7692 0.3504 0.3726 0 0.6901 0.00E+00 0.6642 0.00E+00
9 -0.049437739 0.966330792 14412 484 673 14667 341 568 156 987 0 1.029 1.037 1.004 0.968 1.039 0.9359 0.9359 0.8526 0.859 0.4098 0.4356 0 0.7776 0.00E+00 0.7496 0.00E+00
10 -0.297156798 0.813869833 9800 476 640 11718 331 529 156 1089 0 1.221 1.224 1.197 0.985 1.218 0.9872 0.9872 0.9615 0.9808 0.2512 0.2663 0 0.9092 0.00E+00 0.912 0.00E+00
11 -0.369829217 0.773888465 9486 476 722 11900 328 615 156 1057 0 1.284 1.309 1.241 0.9844 1.265 0.9038 0.9038 0.7756 0.8013 0.384 0.4062 0 0.7224 0.00E+00 0.7364 0.00E+00
12 -0.318294145 0.802032516 11100 464 729 13508 327 636 156 1093 0 1.239 1.219 1.241 0.9864 1.262 1 1 0.9936 0.9936 0.233 0.2475 0 0.9332 0.00E+00 0.9378 0.00E+00
13 -0.039322218 0.973130072 46141 463 695 46979 324 644 156 1015 0 1.021 1.002 0.9914 0.9871 1.004 1 0.9872 0.891 0.9487 0.3127 0.2975 0 0.8649 0.00E+00 0.8783 0.00E+00
14 -0.04915092 0.966522925 44243 466 506 45334 315 415 156 984 0 1.028 1.002 0.9934 0.9904 1.003 0.9808 0.9872 0.9038 0.9295 0.3439 0.3242 0 0.9042 0.00E+00 0.9096 0.00E+00
15 -0.06539434 0.955701798 43136 477 528 44677 311 417 156 1038 0 1.04 1.003 1.004 0.9937 1.011 0.9936 0.9808 0.859 0.9615 0.348 0.3311 0 0.8897 0.00E+00 0.9134 0.00E+00
16 -0.283644179 0.821528539 8262 462 490 9740 303 353 156 1037 0 1.21 1.186 1.211 0.992 1.223 0.9167 0.8782 0.7051 0.7308 0.3754 0.4127 0 0.6936 0.00E+00 0.6862 0.00E+00
17 -0.309101355 0.807159349 11605 443 457 14035 290 318 156 987 0 1.231 1.208 1.141 0.9702 1.182 0.8974 0.891 0.75 0.8077 0.4015 0.4356 0 0.7453 0.00E+00 0.7628 0.00E+00
18 -0.319775846 0.801209222 9532 453 467 11542 279 307 156 1012 0 1.241 1.241 1.239 0.9944 1.247 0.9231 0.9551 0.7628 0.8141 0.3839 0.4123 0 0.7603 0.00E+00 0.7475 0.00E+00
19 -0.190786505 0.87614422 8500 460 475 9397 276 302 156 1037 0 1.134 1.136 1.125 0.9935 1.133 0.9808 0.9359 0.8718 0.8718 0.3083 0.3215 0 0.867 0.00E+00 0.842 0.00E+00
20 -0.182132102 0.881415808 8237 459 467 9042 271 285 156 1009 0 1.128 1.124 1.107 0.9913 1.118 0.9936 0.9808 0.9551 0.9487 0.3196 0.3359 0 0.9492 0.00E+00 0.9215 0.00E+00

Total number of rows: 16928

Table truncated, full table size 2857 Kbytes.




Supplementary data files not provided

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