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Sample GSM3132405 Query DataSets for GSM3132405
Status Public on Jul 06, 2018
Title Arabidopsis heso1-1 miR1510
Sample type SRA
Source name Leaf
Organism Arabidopsis thaliana
Characteristics developmental stage: 2-week old
tissue: leaf
ecotype: heso1-1
Growth protocol Arabidopsis and mung bean were grown in a growth chamber or greenhouse  with a light cycle of 16 h light / 8 h dark, at 22 to 23 °C. Pigeon pea plants were grown  in a greenhouse with a light cycle of 16 h light / 8 h dark, at 24 to 28 °C. 
Extracted molecule total RNA
Extraction protocol Total RNA samples in this study were purified from plant materials using PureLink Plant RNA Reagent.
Library strategy ncRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina NextSeq 500
Data processing The adapter sequences were trimmed from raw sequence reads, and then the small RNA sequences were trimmed to the lengths between 15 and 34 nucleotides. Small RNA reads were mapped to the Pigeon pea, mung bean, and Arabidopsis genomes.
Genome_build: Pigeon pea Cc1.0; mung bean Vr1.0; Arabidopsis thaliana TAIR10.
Supplementary_files_format_and_content: Tag count file
Submission date May 04, 2018
Last update date Jul 07, 2018
Contact name Blake C. Meyers
Phone 314-587-1422
Organization name Donald Danforth Plant Science Center
Lab Meyers lab
Street address 975 N Warson Road
City St. Louis
State/province MO
ZIP/Postal code 63132
Country USA
Platform ID GPL19580
Series (1)
GSE114075 Biogenesis of a young, 22-nt microRNA in Phaseoleae species by precursor-programmed uridylation
BioSample SAMN09078333
SRA SRX4045483

Supplementary file Size Download File type/resource
GSM3132405_miR1510_heso1-1_chopped.txt.gz 15.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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