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Sample GSM315705 Query DataSets for GSM315705
Status Public on Jan 26, 2009
Title MIFS_case_5
Sample type RNA
Source name Case 5
Organism Homo sapiens
Characteristics RNA from fresh frozen tumor biopsy
Biomaterial provider Dept of Clinical Genetics, Lund University Hospital, Sweden
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen tumor biopsies using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and RNeasy (Qiagen, Valencia, CA, USA) according to the manufacturers' instructions.
RNA quality and concentration were measured using an Agilent 2100 bioanalyzer and Nanodrop ND-1000, respectively. cDNA was generated with the GeneChip® Whole Transcript (WT) cDNA Synthesis and Amplification Kit (Affymetrix) using 300 ng total RNA. Amplified cDNA was fragmented and end labeled using the GeneChip® WT Terminal Labelling Kit (Affymetrix). Subsequently, the fragmented and biotinylated cDNA was hybridized to the GeneChip® Human Gene 1.0 ST Arrays (Affymetrix). The arrays were washed and stained on a GeneChip® Fluidics Station 450 (Affymetrix) according to the manufacturer’s recommendations.
Label biotin
Label protocol GeneChip® WT Terminal Labelling Kit (Affymetrix Inc, Santa Clara, CA, USA)
Hybridization protocol Arrays were hybridized and washed according to the manufacturer's specifications (Affymetrix Inc., Santa Clara, CA, USA)
Scan protocol Arrays were scanned in a GeneChip® Scanner 3000 (Affymetrix Inc., Santa Clara, CA, USA)
Description Genome-wide gene expression profiling of MIFS using the Affymetrix GeneChip® Human Gene 1.0 ST Arrays (Affymetrix Inc., Santa Clara, CA, USA)
Data processing Image analysis was performed using GeneChip® Operating Software (GCOS, Affymetrix). Expression data was normalized, background corrected and summarized using the RMA algorithm implemented in the Affymetrix Expression Console™ version 1.0 software.
Submission date Aug 27, 2008
Last update date Jan 26, 2009
Contact name Karolin H Nord
Organization name Lund University
Department Dept of Clinical Genetics
Street address BMC C13
City Lund
ZIP/Postal code SE-22184
Country Sweden
Platform ID GPL6244
Series (2)
GSE12592 Gene expression analysis of myxoinflammatory fibroblastic sarcoma, and morphologically similar lesions
GSE12595 Two genetic pathways in myxoinflammatory fibroblastic sarcoma and morphologically similar lesions

Data table header descriptions
VALUE RMA-normalized intensities

Data table
7892501 5.173068
7892502 6.491841
7892503 2.899275
7892504 8.295075
7892505 2.795437
7892506 3.2294
7892507 4.816465
7892508 6.224117
7892509 10.54287
7892510 5.318713
7892511 4.196647
7892512 6.806345
7892513 3.46213
7892514 10.5828
7892515 8.938506
7892516 5.011152
7892517 5.908856
7892518 1.783789
7892519 5.119192
7892520 9.234921

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM315705.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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