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Sample GSM316734 Query DataSets for GSM316734
Status Public on Sep 03, 2008
Title Xenopus borealis Testis, biological rep 3
Sample type RNA
Source name Xenopus borealis, sexually mature testis
Organism Xenopus borealis
Characteristics Adult male Xenopus borealis (Kenya)
Treatment protocol Animal was immersed in MS222 and gonad was immediately extracted
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
Hybridization protocol Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description 1 of 3 biological replicates of gene expression data from sexually mature testis.
Data processing Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.borealis brain
Submission date Aug 29, 2008
Last update date Sep 02, 2008
Contact name Frederic Chain
Organization name McMaster University
Department Biology
Lab LSB 324
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S4K1
Country Canada
Platform ID GPL1318
Series (1)
GSE12625 Expression data for a study on cross-species hybridization on single-species microarrays

Data table header descriptions
VALUE RMA normalized (log2) using RMA express using "XB+XL perfect match probemask"

Data table
Xl.1.1.S1_s_at 12.90737107
Xl.10012.1.A1_at 7.584385149
Xl.10032.1.A1_s_at 9.294157211
Xl.10043.1.A1_at 6.036917796
Xl.10043.2.S1_at 3.955864776
Xl.10054.1.S1_at 9.090299653
Xl.10087.1.A1_at 9.681999369
Xl.10104.1.A1_at 6.1490619
Xl.10118.1.S1_at 5.604785672
Xl.1013.1.S1_at 5.972671588
Xl.10142.1.S1_at 7.906927101
Xl.1015.1.S1_at 8.740529921
Xl.10170.1.A1_at 7.962661038
Xl.10191.1.S1_at 9.442154493
Xl.10208.1.S1_at 6.583339684
Xl.10222.1.A1_at 5.786716216
Xl.10227.1.A1_at 6.947293935
Xl.10230.1.A1_at 8.345293562
Xl.10251.1.A1_at 5.643607938
Xl.10287.1.S1_at 8.671746815

Total number of rows: 2143

Table truncated, full table size 59 Kbytes.

Supplementary file Size Download File type/resource
GSM316734.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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