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Sample GSM31782 Query DataSets for GSM31782
Status Public on Nov 01, 2004
Title Fig5 HeLa rep1
Sample type RNA
 
Source name HeLa cells
Organism Homo sapiens
Extracted molecule total RNA
 
Description Figure 5, A novel method for microarray-based, high-throughput, gene expression profiling of microRNAs. Nature Methods, Nov 2004
Keywords = microRNA
 
Submission date Oct 04, 2004
Last update date May 27, 2005
Contact name Don Baldwin
E-mail(s) dbaldwin@mail.med.upenn.edu
Phone 215-898-3675
Fax 215-898-4227
URL http://www.med.upenn.edu/microarr
Organization name University of Pennsylvania
Department Pathology and Lab Medicine
Lab Penn Microarray Facility
Street address 277 John Morgan, 3620 Hamilton Walk
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL1464
Series (1)
GSE1821 RAKE microRNA

Data table header descriptions
ID_REF
Block subarray (print pin)
Column subarray column
Row subarray row
Name oligo probe name
X scan X coordinate
Y scan Y coordinate
Dia. spot diameter
F532 Median foreground pixels median, detection at wavelength 532nm
F532 Mean foreground pixels mean
F532 SD foreground pixels standard deviation
F532 CV foreground pixels coefficient of variance
B532 background at wavelength 532nm
B532 Median background pixels median
B532 Mean background pixels mean
B532 SD background pixels standard deviation
B532 CV background pixels coefficient of variance
% > B532+1SD percentage foreground pixels greater than background plus one standard deviation
% > B532+2SD percentage foreground pixels greater than background plus two standard deviations
F532 % Sat. percentage foreground pixels at detector saturation
F Pixels number of foreground pixels
B Pixels number of background pixels
Circularity GenePix Pro circularity score
Sum of Medians (532/1) sum of medians
Sum of Means (532/1) sum of means
VALUE background corrected median signal
F532 Mean - B532 background corrected mean signal
F532 Total Intensity total of all foreground pixel fluorescence
SNR 532 signal to noise ratio
Flags GenePix Pro flag values

Data table
ID_REF Block Column Row Name X Y Dia. F532 Median F532 Mean F532 SD F532 CV B532 B532 Median B532 Mean B532 SD B532 CV % > B532+1SD % > B532+2SD F532 % Sat. F Pixels B Pixels Circularity Sum of Medians (532/1) Sum of Means (532/1) VALUE F532 Mean - B532 F532 Total Intensity SNR 532 Flags
100019 1 1 1 ath-miR158 7170 7900 150 1665 1649 130 7 1923 1923 1920 267 13 0 0 0 156 1272 100 -258 -274 -258 -274 257220 -1.015 -50
101285 1 1 2 mmu-mir-23b 7170 8300 150 1599 1599 166 10 1899 1899 1886 202 10 1 0 0 156 1272 100 -300 -300 -300 -300 249464 -1.421 -50
101021 1 1 3 mmu-mir-125b 7170 8740 200 2600 2608 161 6 1963 1963 2013 244 12 99 87 0 194 2249 0 637 645 637 645 505936 2.439 0
100751 1 1 4 miR-106a 7140 9120 200 2953 3027 328 10 1963 1963 1979 159 8 100 99 0 254 2285 0 990 1064 990 1064 768841 6.591 0
100475 1 1 5 hsa-mir-211 7170 9500 150 1562 1558 106 6 2011 2011 1997 213 10 0 0 0 156 1272 100 -449 -453 -449 -453 243084 -2.061 -50
100115 1 1 6 ath-miR319 7170 9900 150 2114 2152 270 12 2019 2019 2044 197 9 30 16 0 156 1272 100 95 133 95 133 335642 0.548 -50
101381 1 1 7 rno-miR-325 7170 10300 150 1483 1462 159 10 1918 1918 1920 223 11 0 0 0 156 1272 100 -435 -456 -435 -456 228096 -2.054 -50
101117 1 1 8 mmu-mir-145 7170 10700 150 1904 1870 213 11 1957 1957 1965 532 27 0 0 0 156 1272 100 -53 -87 -53 -87 291795 -0.179 -50
100020 1 2 1 ath-miR158 7570 7900 150 1600 1617 198 12 2024 2024 2011 289 14 0 0 0 156 1272 100 -424 -407 -424 -407 252259 -1.363 -50
101286 1 2 2 mmu-mir-23b 7570 8300 150 1602 1605 173 10 1878 1878 1868 150 8 1 0 0 156 1272 100 -276 -273 -276 -273 250360 -1.753 -50
101022 1 2 3 mmu-mir-125b 7530 8680 170 2498 2515 148 5 1886 1886 1899 146 7 100 99 0 151 1483 0 612 629 612 629 379768 4.219 0
100752 1 2 4 miR-106a 7530 9090 180 2667 2694 172 6 1955 1955 1993 612 30 83 2 0 136 1870 5 712 739 712 739 366404 1.145 0
100476 1 2 5 hsa-mir-211 7570 9500 150 1545 1556 128 8 1942 1942 1915 171 8 0 0 0 156 1272 100 -397 -386 -397 -386 242762 -2.099 -50
100116 1 2 6 ath-miR319 7520 9900 120 2948 2947 159 5 2004 2004 2076 1224 58 6 0 0 44 832 14 944 943 944 943 129677 0.712 0
101382 1 2 7 rno-miR-325 7570 10300 150 1442 1460 169 11 1900 1900 1898 163 8 0 0 0 156 1272 100 -458 -440 -458 -440 227732 -2.687 -50
101118 1 2 8 mmu-mir-145 7570 10700 150 1896 1898 113 5 1894 1894 1898 144 7 9 1 0 156 1272 100 2 4 2 4 296103 0 -50
100403 1 3 1 hsa-mir-197 7970 7900 150 1321 1363 271 19 1990 1990 1916 300 15 0 0 0 156 1272 100 -669 -627 -669 -627 212650 -1.843 -50
100043 1 3 2 ath-miR161 7970 8300 150 1493 1474 171 11 1852 1852 1843 188 10 0 0 0 156 1272 100 -359 -378 -359 -378 229878 -1.963 -50
101309 1 3 3 mmu-mir-30e 7970 8700 150 1803 1799 191 10 1907 1907 1927 179 9 6 0 0 156 1272 100 -104 -108 -104 -108 280672 -0.715 -50
101045 1 3 4 mmu-mir-132 7970 9100 150 1532 1551 187 12 1877 1877 1862 178 9 1 1 0 156 1272 100 -345 -326 -345 -326 242033 -1.747 -50

Total number of rows: 1536

Table truncated, full table size 200 Kbytes.




Supplementary data files not provided
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