|
| Status |
Public on Sep 16, 2008 |
| Title |
Chromosome 12 Replacement alpha-type Cell Replicate 2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
S. cerevisiae chromosome 12 replacement alpha-type cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: W303；S. cerevisiae chromosome 12 was replaced by S. bayanus chromosome 12
|
| Treatment protocol |
S. bayanus were crossed to S. cerevisiae to generate F1 hybrid cells. The hybrid diploids were induced to sporulate and viable spores were backcrossed to S. cerevisiae to construct chromosome replacement lines
|
| Growth protocol |
Yeast cells were grown in YPD medium, at 30 degree
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit
|
| Label |
Cy5
|
| Label protocol |
fragmentation protocol：4.5 µg total genomic DNA were digested by 1 µl Dnase I (0.05 U) at 37 degree for 5 minutes and at 100 degree, 15 minutes for enzyme inactivation. The label protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
| |
|
| Channel 2 |
| Source name |
S. cerevisiae alpha-type cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: W303
|
| Growth protocol |
Yeast cells were grown in YPD medium, at 30 degree
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Yeast genomic DNA was extracted using QIAGEN Genomic-tip 100/G kit
|
| Label |
Cy3
|
| Label protocol |
fragmentation protocol：4.5 µg total genomic DNA were digested by 1 µl Dnase I (0.05 U) at 37 degree for 5 minutes and at 100 degree, 15 minutes for enzyme inactivation. The label protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
| |
|
| |
| Hybridization protocol |
The hybridization protocol was adapted from Microarray Core Facility, Insititute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html)
|
| Scan protocol |
Arrays were scanned for F635(cy5) and F532(Cy3) fluoresence intensities using AXON 4000B ( Molecular Device , sunnyvale , CA )
|
| Description |
The S. cerevisiae hybrid karyotypes were analyzed by array-CGH to identify small regions of duplication or homeologous chromosomal exchange occurring during the strain construction.
|
| Data processing |
Subtracted background. Dividing by ch2 (control) was used to transform data (Agilent GeneSpring 7.3.1 software)
|
| |
|
| Submission date |
Sep 15, 2008 |
| Last update date |
Sep 15, 2008 |
| Contact name |
Jun-Yi Leu |
| E-mail(s) |
jleu@imb.sinica.edu.tw
|
| Organization name |
Academia Sinica
|
| Department |
Insititute of Molecular Biolody
|
| Street address |
128 Academia Road, Section 2, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL7305 |
| Series (2) |
| GSE12776 |
Karyotype analysis of S. cerevisiae chromosome replacement lines - alpha-type cells |
| GSE12785 |
Karyotype analysis of S. cerevisiae chromosome replacement lines |
|
