|
| Status |
Public on Apr 23, 2019 |
| Title |
acinar-to-ductal metaplasia, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
acinar-to-ductal metaplasia
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6/J wildtype
cell type: acinar-to-ductal metaplasia
replicate: 2
|
| Treatment protocol |
acinar-to-ductal-metaplasia was additionally treated with 50 ng/ml Tgf-alpha.
|
| Growth protocol |
embryonic acinar cells and adult acinar cells were freshly isolated by collagen-digestion and directly used for RNA extraction. acinar-to-ductal-metaplasia was cultivated in 3D-collagen for 7 days. Low-passage tumor cells were grown in standard medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNeasy Mini Kit from Qiagen according manufactures protocol.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
3D_2
|
| Data processing |
R/Bioconductor, rma normalization, default parameters
|
| |
|
| Submission date |
Jun 22, 2018 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center, Bioinformatics
|
| Street address |
Großhadener Str. 9
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE116150 |
Dynamic changes in the epigenetic landscape are mandatory for acinar cell reprogramming and pancreatic carcinogenesis [microarray] |
| GSE116152 |
Dynamic changes in the epigenetic landscape are mandatory for acinar cell reprogramming and pancreatic carcinogenesis |
|
