NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3211438 Query DataSets for GSM3211438
Status Public on Apr 23, 2019
Title acinar-to-ductal metaplasia, replicate 3
Sample type RNA
 
Source name acinar-to-ductal metaplasia
Organism Mus musculus
Characteristics strain: C57Bl6/J wildtype
cell type: acinar-to-ductal metaplasia
replicate: 3
Treatment protocol acinar-to-ductal-metaplasia was additionally treated with 50 ng/ml Tgf-alpha.
Growth protocol embryonic acinar cells and adult acinar cells were freshly isolated by collagen-digestion and directly used for RNA extraction. acinar-to-ductal-metaplasia was cultivated in 3D-collagen for 7 days. Low-passage tumor cells were grown in standard medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with RNeasy Mini Kit from Qiagen according manufactures protocol.
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description 3D_3
Data processing R/Bioconductor, rma normalization, default parameters
 
Submission date Jun 22, 2018
Last update date Apr 23, 2019
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL16570
Series (2)
GSE116150 Dynamic changes in the epigenetic landscape are mandatory for acinar cell reprogramming and pancreatic carcinogenesis [microarray]
GSE116152 Dynamic changes in the epigenetic landscape are mandatory for acinar cell reprogramming and pancreatic carcinogenesis

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
17200001 3.79
17200003 3.99
17200005 2.73
17200007 2.3
17200009 2.71
17200011 2.54
17200013 3.02
17200015 3.72
17200017 2.8
17200019 2.17
17200021 2.53
17200023 4.47
17200025 3.95
17200027 3.89
17200029 2.53
17200031 2.19
17200033 2.68
17200035 2.62
17200037 2.72
17200039 2.36

Total number of rows: 41345

Table truncated, full table size 561 Kbytes.




Supplementary file Size Download File type/resource
GSM3211438_3D_3.CEL.gz 8.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap